Fluorescence Spectroscopy


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Dual-FL - The world's fastest Fluorometer


HORIBA Scientific combined the world’s only CCD based benchtop spectrofluorometer with a UV-VIS spectrophotometer and created the Dual-FL.

The Dual-FL features spectral rates as fast as 80,000 nm/s, and a water Raman S/N sensitivity greater than 20,000:1 RMS.

Collect incredibly rapid kinetics data, or complete excitation emission matrix (EEMs) in seconds.

And, it also has a built-in UV-VIS spectrophotometer. Use it as your lab UV-VIS, or collect simultaneous dual-mode fluorescence and absorbance data to correct inner-filter effects in your spectra.



  • The only true simultaneous absorbance-fluorescence system available

  • TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer

  • Corrected UV-VIS absorbance detection path for stability and accuracy

  • Double grating excitation monochromator for superior stray light rejection

  • Matching bandpass for absorbance and fluorescence spectra

  • Automatic sample changer option (two or four position)

  • Compatible with flow cells and titrator


  • Optimized experiment set-up menus minimize user confi guration time

  • Complete NIST-traceable corrected fluorescence spectra automatcally generated

  • Spectral and kinetic analysis tools for both absorbance and fluorescence data

  • Methods and batch protocols for automating multiple sample measurement

'How Fast is Fast?'

Vyrobeno HORIBA


Fluorescence Hardware

Light source

150W ozone free vertically mounted xenon arc lamp

Excitation range

200 - 800 nm (Dual-FL UV)

Excitation bandpass

5 nm

Excitation monochromator

Subtractive double monochromator

Excitation gratings

1200 gr/mm, 250 nm blaze

Excitation wavelength accuracy

±1 nm

Emission range

250 - 800 nm

Emission bandpass

5 nm

Emission spectrograph

Fixed, aberration-corrected 140 mm focal length

Emission grating

405 gr/mm; 250 nm blaze

Emission detector

TE-cooled back-illuminated CCD

Emission integration time

5 ms minimum

Hardware pixel binning

0.41, 0.82, 1.64, 3.28 nm/pixel

CCD gain options

4.5 e-/cts in medium gain, 9 e-/cts in low gain


Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)


33 kg (72 lbs)


LWH (618 x 435 x 336 mm); (24" x 17" x 13")

Absorbance Hardware

Scanning range

230 - 800 nm (optical)


5 nm

Slew speed

Max 500 nm/s

Optical system

Corrected single beam


Si photodiode

Wavelength accuracy

±1 nm

Wavelength repeatability

+/- 0.5 nm

Photometric accuracy

±0.01 AU from 0 to 2A

Photometric stability

<0.002 AU per h

Photometric repeatability

 +/- 0.002 AU (0 to 1 AU)

Stray light

<1% measured with KI standard