Fluorescence – Water Treatment Plant Analyzer - Aqualog

A better, faster and economical method for monitoring organics

Aqualog® is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.

Aqualog now offers new deep UV absorbance and fluorescence excitation from 200 nm, in addition to a fully automated sample queue tool to facilitate continuous data acquisition, analysis and export for immediate multivariate analysis with the Solo™ package from our partner, Eigenvector Research, Inc.

Segment: Scientific
Division: Molecular and Microanalysis
Base product
Manufacturing Company: HORIBA Scientific


  • The only true simultaneous absorbance-fluorescence system available
  • TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer
  • Corrected UV-VIS absorbance detection path for stability and accuracy
  • Double grating excitation monochromator for superior stray light rejection
  • Matching bandpass for absorbance and fluorescence spectra
  • Automatic sample changer option (2 or 4 position)
  • Compatible with flow cells and titrator


  • Optimized experiment set-up menus minimize user configuration time
  • Complete NIST-traceable corrected fluorescence spectra automatically generated
  • Spectral and kinetic analysis tools for both absorbance and fluorescence data
  • Methods and batch protocols for automating multiple sample measurement

'How Fast is Fast?'

Click here for the Aqualog 3.6.11 Patch.
Please Note: Please select to save the file.Release Notes.

NEW A-TEEM™ technology

A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!

All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.

Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.

NEW Aqualog® Datastream Dashboard


  • Seamless integration with Aqualog
  • Convenient HTML-based interface
  • Push-button method operation
  • Simple administrator level controls for calibration and method development


  • Easy access through internet or intranet
  • Dashboard shows the latest readings, time series and tables for trends and analysis
  • WTP can upload their own independent data

Aqualog Datastream Dashboard is powered by Solo_Predictor software from
Eigenvector Research, Incorporated

NEW Automatic Sipper Accessory

New for our Aqualog®  A-TEEM™ spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.

The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection.  It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.

When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources.

Fluorescence Hardware

Choice of Light source

Standard: 150W ozone-free vertically mounted xenon arc lamp

Extended-UV: 150W vertically mounted xenon arc lamp

Excitation range

230 nm to upper limit of emission detector

200 nm to upper limit of emission detector

Excitation bandpass

5 nm

Excitation monochromator

Subtractive double monochromator

Excitation gratings

1200 gr/mm, 250 nm blaze

Excitation wavelength accuracy

±1 nm

Choice of Detector



Emission range

250-620 nm

250-800 nm

Emission grating

405 gr/mm; 250 nm blaze

285 gr/mm; 350 nm blaze

Hardware pixel binning

0.41, 0.82, 1.64, 3.28 nm/pixel

0.58, 1.16, 2.32, 4.64 nm/pixel

Emission bandpass

5 nm

Emission spectrograph

Fixed, aberration-corrected 140 mm focal length

Emission detector

TE-cooled back-illuminated CCD

Emission integration time

5 ms minimum


CCD gain options

2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain


Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)


32.72 kg (72 lbs)


LWH (618 x 435 x 336 mm); (24" x 17" x 13")

Holistic Analysis of Mammalian Cell Proliferation using Fluorescence Spectroscopy
Holistic Analysis of Mammalian Cell Proliferation using Fluorescence Spectroscopy
Noninvasive In-Vivo Determination of Sunscreen-UVA Protection Factors
Noninvasive In-Vivo Determination of Sunscreen-UVA Protection Factors
Spectroscopic Analysis of Red Wines with Aqualog
Spectroscopic Analysis of Red Wines with Aqualog
EPA Stage 2 Disinfection Compliance with Aqualog
EPA Stage 2 Disinfection Compliance with Aqualog
Fast & Non-invasive Determination of Skin State
Fast & Non-invasive Determination of Skin State
Insulin Structure and Stability Assessment
Insulin Structure and Stability Assessment
Aqualog for Water Treatment Organics Monitoring Brochure
Size 2.99 MB
Aqualog Sipper Specification Sheet PDF
Size 1.44 MB
Aqualog Datastream Dashboard PDF
Size 1.61 MB
Aqualog Brochure PDF
Size 1.19 MB
Aqualog Spectroscopic Analysis of Red Wines Application Note PDF
Size 1.78 MB
A-TEEM, a new molecular fingerprinting technique Technote
Size 0.9 MB


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