Multiparticle Analysis by Raman Microscopy

Multiparticle Analysis by Raman Microscopy

There are multiple circumstances where characterization of a collection of particles has value to analysts. In some environments, materials are plagued by particulate contamination that impacts product quality. In the case of small-molecule pharmaceuticals, the solid form of the active pharmaceutical ingredient has to be controlled because of its impact on stability, bioavailability, and intellectual property protection. Raman analysis of relatively large areas with sparsely dispersed particles is now integrated with particle statistics. Results of some simple studies are shown in this column.

Published in February 2015 issue

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Raman Spectroscopy and Microscopy Enable Life Science Discoveries

Raman Spectroscopy and Microscopy Enable Life Science Discoveries

The first article in this two-part series looked at Raman spectroscopy applications in tumor surgery, endoscopy and bacterial identification. This second part examines applications in cell sorting, laser trapping, biology probing and more.

Published in BioPhotonics, February/March 2015 issue

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Raman Spectroscopy and Microscopy: Solving Outstanding Problems in the Life Sciences

Raman Spectroscopy and Microscopy: Solving Outstanding Problems in the Life Sciences

This first article in a two-part series will examine Raman spectroscopy applications in tumor surgery, endoscopy, bacterial identification and more. The second part will cover applications in cell sorting, laser trapping and probing biology.

Published in BioPhotonics, January 2015 issue

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Three-Dimensional Raman Imaging of Ion-Exchanged Waveguides

Three-Dimensional Raman Imaging of Ion-Exchanged Waveguides

Segmented channel waveguides have been fabricated in single-crystal KTiOPO4 through a topotactic process of partial cation exchange. The ion-exchanged waveguides maintain the high nonlinear susceptibility of KTiOPO4 to function as frequency doubling laser light sources. We applied three-dimensional Raman imaging to understand and characterize the changes to the chemical bonding and crystalline structure as well as measure the volumetric structure of waveguide segments.

Published in Spectroscopy, December 2014

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Raman Spectra of Metal Oxides

Raman Spectra of Metal Oxides

Metal oxides often occur in crystals where there are no molecular units. In contrast to Raman analysis of organic compounds in which there are molecular vibrating units with functional subunits, many of whose vibrations are isolated from the remainder of the molecule, the analysis of spectra of oxides requires understanding how atoms move in crystal lattices. These oxides occur in diverse materials such as paint and ceramic pigments, corrosion films, catalysts, and minerals. This column installment attempts to introduce analysts who often work with organic materials to the concepts that need to be understood in analyzing these materials.

Published in Spectroscopy, October 2014

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Headspace Raman Spectroscopy

Headspace Raman Spectroscopy

We examine vapor-phase Raman spectroscopy through the acquisition of spectra from gas molecules confined to the headspace of sealed containers. Studying the Raman spectra of the liquid and vapor phases of compounds with different functional groups, degrees of hydrogen bonding, and polarity provides insight into the energetics of molecular interactions.

Published in Spectroscopy, September 2014

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Raman Microscopy for Detecting Counterfeit Drugs — A Study of the Tablets Versus the Packaging

Raman Microscopy for Detecting Counterfeit Drugs — A Study of the Tablets Versus the Packaging

With the increasing proliferation of counterfeit drug products, there is an incentive to screen drugs for legitimacy. One method is to examine the tablet itself, which is usually a destructive operation. Another method that has been explored is to characterize the packaging, which enables nondestructive screening of the product. Raman microscopy has been found to be a useful tool, and it is often the tool of choice for these measurements.

Published in Spectroscopy, June 2014

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High-Speed TERS Imaging: The Latest Achievements in nano-Raman Spectroscopy

High-Speed TERS Imaging: The Latest Achievements in nano-Raman Spectroscopy

This article presents developments in tip-enhanced Raman spectroscopy (TERS) that make possible nanoscale imaging of chemical and physical properties of graphene and other carbon species: Advances in near-field optical probes now provide reliable nanoscale spectroscopy solutions for academic and industrial researchers.

Published in Spectroscopy, June 2014

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Practical Group Theory and Raman Spectroscopy, Part II: Application of Polarization

Practical Group Theory and Raman Spectroscopy, Part II: Application of Polarization

In this second installment of a two-part series we present polarized Raman spectra and discuss the association of the symmetry species of the normal vibrational mode and the depolarization ratio of Raman scattering. We discuss those aspects of molecular symmetry and Raman polarization rules that can be applied with normal Raman instrumentation. Materials include liquids, single crystals, and polycrystalline compounds.

Published in Spectroscopy, March 2014

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Practical Group Theory and Raman Spectroscopy, Part I: Normal Vibrational Modes

Practical Group Theory and Raman Spectroscopy, Part I: Normal Vibrational Modes

Group theory is an important component for understanding the fundamentals of vibrational spectroscopy. The molecular or solid state symmetry of a material in conjunction with group theory form the basis of the selection rules for infrared absorption and Raman scattering. Here we investigate, in a two-part series, the application of group theory for practical use in laboratory vibrational spectroscopy.

Published in Spectroscopy, February 2014

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Considerations of Grating Selection in Optimizing a Raman Spectrograph

Considerations of Grating Selection in Optimizing a Raman Spectrograph

The performance of a Raman spectrograph for a particular application will depend, among other things, on its sensitivity and spectral resolution. The sensitivity will determine how long it will take to record a spectrum with a given signal-to-noise ratio.

Published in Spectroscopy, October 2013

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Raman Imaging of Silicon Structures

Raman Imaging of Silicon Structures

Here we examine what is commonly called a Raman image and discuss how it is rendered. We consider a Raman image to be a rendering as a result of processing and interpreting the original hyperspectral data set.

Published in Spectroscopy, September 2013

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Resonance Enhancement of Raman Spectroscopy: Friend or Foe?

Resonance Enhancement of Raman Spectroscopy: Friend or Foe?

The presence of electronic transitions in the visible part of the spectrum can provide enormous enhancement of the Raman signals, if these electronic states are not luminescent. In some cases, the signals can increase by as much as six orders of magnitude.

Published in Spectroscopy, June 2013

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Classical or Transmission Raman, SERS or TERS: Which Raman Spectroscopy Technique Is Right for You?

Classical or Transmission Raman, SERS or TERS: Which Raman Spectroscopy Technique Is Right for You?

This article explains different Raman techniques are compared with respect to their main field of applications, possibilities, advantages, difficulties, and limits.

Published in Spectroscopy, June 2013

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Raman for Oil Shale Exploration

Raman for Oil Shale Exploration

Discussion and examples of the practical application of Raman micro-spectroscopy for the analysis of materials directly related to oil shale exploration.

Published in Spectroscopy, March 2013

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Raman Microscopy Analysis of Molecular Orientation in Organic Fibres

Raman Microscopy Analysis of Molecular Orientation in Organic Fibres

The processes involved in creating fibers from organic polymers - either man-made or natural - have the potential to orient the molecules in the fibres.  Examination of a number of fibres confirms that orientation is a more or less ubiquitous phenomenon that can be detected and quantified by Raman microscopy.

Published in Spectroscopy, February 2013

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From Micro– to Macro–Raman Spectroscopy:Solar Silicon for a Case Study

From Micro– to Macro–Raman Spectroscopy:Solar Silicon for a Case Study

The paper explains the use of the Raman spectroscopy to the stress analysis in Silicon based panel solar materials.

Published by InTech Advanced Aspects of Spectroscopy as a chapter of a book "Advanced Aspects of Spectroscopy"., 2012.

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Multivariate data processing of spectral images: The Ugly, the Bad and the True

Multivariate data processing of spectral images: The Ugly, the Bad and the True

The paper demonstrates the difference between 'pretty' and 'true' Raman chemical images, and how to achieve true and pretty Raman chemical images.

Published in Spectroscopy, August 2007

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Raman Imaging: Defining the Spatial Resolution of the Technology

Raman Imaging: Defining the Spatial Resolution of the Technology

Chemical images of polystyrene beads on silicon acquired using Raman mapping and image processing are reviewed. The effects of the objective on the quality of the final image, particularly its magnification and numerical aperture, and the step size of the map, are discussed as well.

Published in Spectroscopy, supplement Raman, June 2006

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Spectroscopic imaging for the life sciences - more than just a pretty picture

It has long been a major goal of scientists to understand the many complex processes taking place within living organisms, ranging from microscopic species such as bacteria and viruses at one extreme, through to the human at the other. This article shows that micro-spectroscopic imaging is a key technique for biological research.

Published in Spectroscopy Europe Life Science supplement, August/September 2005

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Spectroscopy Solutions for Materials Analysis

Spectroscopy Solutions for Materials Analysis

Raman and EDXRF Chemical Imaging for Formulation Process Development and Quality Control Compounds of magnesium and calcium are common components of pharmaceutical formulations.

Spectroscopic imaging can provide a complete understanding of a formulation. This paper compares two spectral imaging techniques - energy dispersive X-Ray Fluorescence (EDXRF) microscopy and Raman microscopy.

Published in Spectroscopy, June 2005

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Biopharma Imaging and Analysis

Biopharma Imaging and Analysis

Advancing Towards a More Detailed Picture of Chemistry

There is a wealth of analytical and imaging techniques available for the measurement of biopharma samples. The samples themselves can be wide ranging and take numerous forms: single cells, tissues, crystals or blended formulations. To study and develop such samples many techniques, including fluorescence microscopy, NIR imaging, AFM and SEM, have been used. Published in European Biopharmaceutical Review, Spring 2005

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Refined Raman Spectroscopy

Refined Raman Spectroscopy

Bringing new insight into industrial processes

Fibre probes and ease of use make Raman spectroscopy systems attractive for monitoring process control in many industries, ranging from pharmaceuticals to petrochemicals. The authors describe the Raman effect and discuss systel and monitoring basics.

Published in Optics & Photonics News, June 05

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A new dimension in cell imaging

A new dimension in cell imaging

Combining chemical and spatial information

There are many microscopic imaging techniques used to investigate the complexities of cell structure, including optical microscopy, confocal laser scanning microscopy, scanning electron microscopy (SEM) and atomic force microscopy (AFM). These techniques provide highly detailed images of the cells, but they fail to characterize the chemistry and composition of the sample under examination. Spectroscopic techniques, however, can do just that, and in particular Raman micro-spectroscopy is fast becoming established for probing cellular biochemistry on the micron scale.

Published in BIOforum Europe, April 2005

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Micro-spectroscopy - shedding light on rock formation

Micro-spectroscopy - shedding light on rock formation

Whilst there are many imaging techniques available to a research scientist, the information which is provided is often only of a visual/topographical nature. What they fail to provide is true compositional (chemical/elemental) analysis of the materials. However, microspectroscopic techniques such as Raman or X-Ray Fluorescence (XRF) can fill this gap, allowing highly detailed images to be generated based upon the sample's material composition.

 Published in Spectroscopy Europe, June 2005

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Art/Archaeology

掲載年

タイトル

著者

掲載誌

2008

On-site Raman analysis of Iznik pottery glazes and pigments

Bersani

J.Raman.Spec.

2006

Raman microscopy and IR spectroscopy of prehistoric paintings from Los Murcielagos cave(Zuheros, Cordoba, Spain)

Hernanz A.

J.Raman.Spec.

2004

Pigments and binders in "Madonna col Bambino e S.Giovannino" by Botticelli investigated by micro-Raman and GC/MS

Colomban

J.Cult.Heritage

BIO

掲載年

タイトル

著者

掲載誌

2011

Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities

Li M.

The ISME Journal

2011

In situ visualization of damaged DNA in human sperm by Raman microspectroscopy

Mallidis

Human Reproduction

2011

Understanding Tissue Specific Compositions of Bioenergy Feedstocks Through Hyperspectral Raman Imaging

Sun L.

Biotechnology and Bioengineering

2010

Biochemical imaging below the diffraction limit-probing cellular membrane related structures by TERS

Bohme

J.Biophoton.

2010

Protein-ligand binding investigated by a single nanoparticle TERS approach

Carrier

Chem.Commun.

2009

Towards a specific characterisation of components on a cell surface-combined TERS-investigations of lipids and human cells

Bohme

J.Raman.Spec.

2008

Tip-enhanced Raman Scattering

Bailo E.

Chem.Soc.Rev.

2008

Cell wall investigations utilizing tip-enhanced Raman scattering

Budich

J.Microscopy

2007

Tip-Enhanced Raman Spectroscopy of Single RNA Strands: Towards a Novel Direct-Sequencing Method

Bailo E.

Angewandte

2007

Raman Microscopy of Freeze-Dried Mouse Eyeball-Slice in Conjunction With the "In Vivo Cryotechnique"

Terada

Microscopy Res. And Technique

2006

Raman imaging to investigate ultrastructure and composition of plant cell walls: distribution of lignin and cellulose in black spruce wood

Agarwal

Planta

2006

New Method for the Microscopic, Nondestructive Acquisition of Ultraviolet Resonance Raman Spectra from Plant Cell Walls

Czaja

Applied Spectroscopy

2006

Combined FT-IR and Raman Spectroscopic Approach for Identification of Multidrug Resistance Phenotype in Cancer Cell Lines

Krishna

Wiley Periodicals,Inc

2006

Analysis of Structural Changes in Bleached Keratin Fibers (Black and White Human Hair) Using Raman Spectroscopy

Kuzuhara

Biopolymers

2006

Raman and Infrared Microspectral Imaging of Mitotic Cells

Matthaus

Applied Spectroscopy

2006

Resonance Raman Evidence of Immobilization of Laccase on Self-Assembled Monolayers of Thiols on Ag and Au Surfaces

Michota-Kaminska

Applied Spectroscopy

2006

Surface- and tip-enhanced Raman scattering of DNA components

Rasmussen

J.Raman.Spec.

2006

Semi-Parametric Estimation in the Compositional Modeling of Multicomponent Systems from Raman Spectroscopic Data

Sowa

Applied Spectroscopy

2005

In vivo chemical investigation of human skin using a confocal Raman fiber optic microprobe

Chrit L.

J.Biomed.Optics

2005

A New Dimension in Cell Imaging

FitzGerald

Bioforum Europe

2005

Characterisation of uterine sarcoma cell lines exhibiting MDR phenotype by vibrational spectroscopy

Krishna

Biochimica et Biophisica Acta

2005

Micro-Raman spectroscopy of mixed cancer cell populations

Krishna

Vibrational Spectroscopy

2005

Composition of bone and apatitic biomaterials as revealed by intravital Raman microspectroscopy

Penel

Bone

Carbon

掲載年

タイトル

著者

掲載誌

2010

An Analytical System for Single Nanomaterials: Combination of Capillary Electrophoresis with Raman Spectroscopy or with Scanning Probe Microscopy for Individual Single-Walled Carbon Nanotube Analysis

Yamamoto

Anal.Chem.

2008

Optical Resolution and Diameter-Based Enrichment of Single-Walled Carbon Nanotubes through Simultaneous Recognition of Their Helicity and Diameter with Chiral Monoporphyrin

Peng X.

J.Phys.Chem.

2006

Graphite surface disorder detection using in situ Raman microscopy

Hardwick

Solid State Ionics

2004

Enhanced Raman microprobe imaging of single-wall carbon nanotubes

Hadjiev

Nanotechnology

2004

Resonant Raman scattering from N-doped and Si-doped diamondlike amorphous carbon films

Yoshikawa

Phys.Rev. B

Chemical

掲載年

タイトル

著者

掲載誌

2007

Complexation of Phenols by Calix[4]arene Diethers in a Low-Permittivity Solvent. Self-Switched Complexation by 25,27-Dibenzyloxycalix[4]arene

Kunsagi-Mate

J.Phys.Chem.

2006

Catalytic oxidation of methanol on Mo/Al2O3 catalyst: An EPR and Raman/infrared operando spectroscopies study

Brandhorst

Catalysis Today

2006

Modified Coin Cells for In Situ Raman Spectroelectrochemical Measurements of LixV2O5 for Lithium Rechargeable Batteries

Burba

Applied Spectroscopy

2006

Study of the corrosion from the printing plates of 'Guan Zi' by Raman spectroscopy

Shi J.

J.Raman.Spec.

2005

In Situ Raman Spectroscopic Observation of Corrosion Reaction of Fe with Na2O2 up to 833 K

Ohtori

Electrochemistry

2004

In Situ Raman Spectroscopy Investigation of the Dissociation of Methane Hydrate at Temperatures Just below the Ice Point

Komai

J.Phys.Chem.

2003

Surface Raman spectroscopic study of NO transformation over Pd-based catalysts

Mamede

Phys.Chem.Chem.Phys.

Semiconductor

掲載年

タイトル

著者

掲載誌

2009

Raman Spectrum Curve Fitting for Estimating Surface Stress Distribution in Single-Crystal Silicon Microstructure

Komatsubara

J.J.Applied Phys.

2007

Signal enhancement in nano-Raman spectroscopy by gold caps on silicon nanowires obtained by vapour-liquid-solid growth

Christiansen

Nanotechnology

2006

Scanning nano-Raman spectroscopy of semiconducting structures

Hartschuh

Proc.SPIE

2006

Investigation of stress-induced defects in shallow trench isolation by cathodoluminescence and Raman spectroscopies

Sugie

J.Applied Phys.

2003

Raman spectroscopy: about chips and stress

Wolf

Spectroscopy-Europe

1999

Stress measurements using ultraviolet micro-Raman spectroscopy

Dombrowski

Appl.Phis.Lett.

Compound Semiconductor

掲載年

タイトル

著者

掲載誌

2006

Raman Scattering in GaN Nanocolumns and GaN/AlN Multiple Quantum Disk Nanocolumns

Sekine

e-J.Surf.Sci.Nanotech.

2006

Photoluminescence and cathodoluminescence analyses of GaN powder doped with Eu

Wu H.

Appl.Phys.Lett.

2006

Spatially resolved Raman spectroscopy evaluation of residual stresses in 3C-SiC layer deposited on Si substrates with different crystallographic orientations

Zhu W.

Applied Surface Science

2005

Micro-Raman study of columnar GaAs nanostructures

Prunici

Phys.Stat.Sol.

2005

Raman scattering from GaP nanowires

Chapelle

The European Physical Journal B

2005

Polarized Raman Confocal Microscopy of Single Gallium Nitride Nanowires

Pauzauskie

J.Am.Chem.Soc.

2004

Raman scattering study of InGaAs/AlAsSb and InGaAs/AlAs/AlAsSb heterostructures

Mozume

Phys.Stat.Sol.

2004

Micro-Raman spectroscopy of Si-,C-,Mg- and Be-implanted GaN layers

Wang

J.Raman.Spec.

1998

Phonon-assisted photoluminescence in wurtzite GaN epilayer

Liu W.

Semicond.Sci.Technol.

Inorganics

掲載年

タイトル

著者

掲載誌

2008

The white colour in Etruscan polychromes on terracotta: Spectroscopic identification of kaolin

Bordignon

J.Cult.Heritage

2006

Inhomogeneous laser heating and phonon confinement in silicon nanowires: A micro-Raman scattering study

Adu

Physical Review

2006

On-site Raman analysis of the earliest known Meissen porcelain and stoneware

Colomban

J.Raman.Spec.

2006

Raman spectroscopy of cobalt ferrite nanocomposite in silica matrix prepared by sol-gel method

da Silva

J.Non-Crystalline Solids

2006

The use of Raman spectrometry to predict the stability of historic glasses

Robinet L.

J.Raman.Spec.

2005

Raman study of nanoparticle-template interaction in a CoFe2O4/SiO2-based nanocomposite prepared by sol-gel method

Pedroza

J.Magnetism and Magnetic Materials

2005

Comparison of UV and Visible Raman Spectroscopy of Bulk Metal Molybdate and Metal Vanadate Catalysts

Tian

J.Phys.Chem.

2005

Characterization of Si nano-polycrystalline films at the nanometer level using resonant Raman scattering

Yoshikawa

J.Applied Phys.

2003

In situ characterization by Raman and IR vibrational spectroscopies on a single instrument: DeNOx reaction over a Pd/gamma-Al2O3 catalyst

Bourdon

Phys.Chem.Chem.Phys.

Mineralogy

掲載年

タイトル

著者

掲載誌

2010

Confocal Micro-Raman Spectroscopy: A Powerful Tool To Identify Natural And Synthetic Emeralds

Huong

Gems and Gemology

2005

Micro-spectroscopy-shedding light on rock formation

FitzGerald

Spectroscopy-Europe

Pharmaceuticals

掲載年

タイトル

著者

掲載誌

2006

Effect of Physical State and Particle Size Distribution on Dissolution Enhamcement of Nimodipine/PEG Solid Dispersions Prepared by Melt Mixing and Solvent Evaporation

Papageorgiou

AAPS

2005

Experimental observation of an interface-controlled pseudomorphic replacement reaction in a natural crystalline pyrochlore

Geisler

Am.Mineralogist

2005

Crystalline Form Information from Multiwell Plate Salt Screening by Use of Raman Microscopy

Kojima T.

Pharm.Res.

Polymer

掲載年

タイトル

著者

掲載誌

2006

Orientation-Insensitive Spectra for Raman Microspectroscopy

Lefevre

Applied Spectroscopy

2006

Interphase Evolution in Polymer Films by Confocal Raman Microspectroscopy

Tomba

Applied Spectroscopy

2005

Surface modification of metallic cardiovascular stents by strongly adhering aliphatic polyester coatings

Jerome

Wiley Intersicence

Others

掲載年

タイトル

著者

掲載誌

2007

The SERS and TERS Effects Obtained by Gold Droplets on Top of Si Nanowires

Becker

Nano Letters

2007

Multivariate Data Processing of Spectral Images: The Ugly, the Bad, and the True

Lee E.

Spectroscopy 

2007

High contrast scanning nano-Raman spectroscopy of silicon

Lee N.

J.Raman.Spec.

2007

Simple model for the polarization effects in tip-enhanced Raman spectroscopy

Ossikovski

Phys.Rev. B

2006

Optical properties and enhancement factors of the tips for apertureless near-field optica

Mehtani

Journal of Optics A

2005

Nano-Raman spectroscopy with side-illumination optics

Mehtani

J.Raman.Spec.

2004

Case Studies in the Application of Raman Microscopy

Adar

Raman Technol.Today'sSpectrosc.

2004

Determination of Spectrometer-Detector Parameters from Calibration Spectra and the Use of the Parameters in Spectrometer Calibrations

Holy A.

Applied Spectroscopy

2002

In-depth analyses by confocal Raman microspectrometry: experimental features and modeling of the refraction effects

Bruneel

J.Raman.Spec.

1996

Micro-Raman study of the longitudinal acoustic modes(LAM) evolution along the transition front in uniaxially stretched HDPE

Pastor

Colloid Polym.Sci.