Surface Plasmon Resonance imaging (SPRi)

SPRi Biochip

Our biochips are suitable for a range of surface chemistries and are not restricted to a single Ligand / Analyte regime. The biochips are easily adapted for specific processes.

We can offer:

  • Pre-functionalized surfaces - ready-to-use
  • Electroaddressing of your molecules onto the biochip - we do it for you
  • Bare gold surfaces

Our Surface Chemistries

SPRi Surface Chemistry

"ready-to-use" surface chemistries for SPRi-Biochip and SPRi-Slide

Our ready-to-use functionalised biochips are based on the SAM (Self Assembled Multilayers) surface chemistry. Different natures of biomolecules, such as proteins, DNA, carbohydrates etc., can be directly immobilised on the surface.

  Immobilization by direct coupling Packages
CS via probes amino group 3 units
CO via probes amino group 3 units
CSe via biotinylated probes on extravidin surface 3 units
COe via biotinylated probes on extravidin surface 3 units
CTg GST-tagged probes on anti-GST antibody derivatized surface CTg 3 units
CH COOH surface chemistry read-to-be activated 3 units

"we do it for you" surface chemistries for SPRi-Biochip and SPRi-Slide

The immobilisation of ligand by electroadressing is based on the electropolymerisation approach. We can provide the conjugates preparation and the electrodeposition service.

Thanks to this specific functionalisation, diffent kinds of biomolecules can be immobilised such as proteins, DNA, carbohydrates etc.

  Immobilization by direct coupling Packages
CPY the immobilization of pyrrole-conjugate1 interaction partner with polypyrrole copolymerization on biochip surface 3 units
CDZ diazonium salts based procedure for the immobilization of aryl diazonium-derivatives2 on the gold chip CDZ 3 units

1 Pyrrolated-conjugates are prepared using pyrrole-NHS.

2 Aryl diazonium-derivatives preparation procedure is published. See publication Protein−Diazonium Adduct Direct Electrografting onto SPRi-Biochip.