Fluorescence Spectroscopy

Aqualog - CDOM measurements made easy

Overview

Aqualog is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.

Features

Hardware

The only true simultaneous absorbance-fluorescence system available
TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer
Corrected UV-VIS absorbance detection path for stability and accuracy
Double grating excitation monochromator for superior stray light rejection
Matching bandpass for absorbance and fluorescence spectra
Automatic sample changer option (2 or 4 position)
Compatible with flow cells and titrator

Software

Optimized experiment set-up menus minimize user confi guration time
Complete NIST-traceable corrected fluorescence spectra automatcally generated
Spectral and kinetic analysis tools for both absorbance and fluorescence data
Methods and batch protocols for automating multiple sample measurement

Manufactured by HORIBA

Specifications

*Fluorescence Hardware*
Light source	150W ozone free vertically mounted xenon arc lamp
Excitation range	240-630 nm
Excitation bandpass	5 nm
Excitation monochromator	Subtractive double monochromator
Excitation gratings	1200 gr/mm, 250 nm blaze
Excitation wavelength accuracy	±1 nm
Emission range	210-620 nm
Emission bandpass	5 nm
Emission spectrograph	Fixed, aberration-corrected 140 mm focal length
Emission grating	405 gr/mm; 250 nm blaze
Emission detector	TE-cooled back-illuminated CCD
Emission integration time	5 ms minimum
Hardware pixel binning	0.41, 0.82, 1.64, 3.28 nm/pixel
CCD gain options	2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain
Sensitivity	Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)
Weight	32.72 kg (72 lbs)
Dimensions	LWH (618 x 435 x 336 mm); (24" x 17" x 13")

*Absorbance Hardware*
Scanning range	230 - 800 nm (optical)
Bandpass	5 nm
Slew speed	Max 500 nm/s
Optical system	Corrected single beam
Detector	Si photodiode
Wavelength accuracy	±1 nm
Wavelength repeatability	+/- 0.5 nm
Photometric accuracy	±0.01 AU from 0 to 2A
Photometric stability	<0.002 AU per h
Photometric repeatability	+/- 0.002 AU (0 to 1 AU)
Stray light	<1% measured with KI standard