Fluorescence Spectroscopy

News

  • HORIBA Scientific Presents Young Fluorescence Investigator Award at Biophysical Show. Read more
  • HORIBA Scientific Announces New Ultima TCSPC lifetime system, the next generation of turnkey TCSPC Fluorescence Lifetime systems...Read more
  • HORIBA Scientific announces the acquisition of the Photon Technology International (PTI) Business... Read more
  • HORIBA Scientific Announces New Generation of Turnkey TCSPC Fluorescence Lifetime Systems...Read more
  • Aqualog CDOM Fluorometer Wins Award as one of Best New Instruments in 2011...Read more
  • HORIBA Scientific and NKT Photonics Introduce the World’s First Commercial Integrated Supercontinuum Powered Spectrofluorometers... Read more
  • Aqualog®, the only simultaneous absorbance and fluorescence system, measures CDOM (colored dissolved organic matter) in water 100 times faster than previous fluorescence methods...Read more
  • World's First 100MHz Picosecond Diode Source With USB Interface and Plug-and-Play Interchangeable Heads ...Read more

Aqualog® - Our compact, Benchtop Fluorometer for CDOM

Overview

Aqualog

NEW A-TEEM Technology

NEW Datastream Dashboard Software

NEW Automatic Sipper Accessory

 

Aqualog is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.

multivariate analysis - AqualogAqualog now offers new deep UV absorbance and fluorescence excitation from 200 nm, in addition to a fully automated sample queue tool to facilitate continuous data acquisition, analysis and export for immediate multivariate analysis with the Solotm

Features

Aqualog

Hardware

  • The only true simultaneous absorbance-fluorescence system available

  • TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer

  • Corrected UV-VIS absorbance detection path for stability and accuracy

  • Double grating excitation monochromator for superior stray light rejection

  • Matching bandpass for absorbance and fluorescence spectra

  • Automatic sample changer option (2 or 4 position)

  • Compatible with flow cells and titrator

Software

  • Optimized experiment set-up menus minimize user confi guration time

  • Complete NIST-traceable corrected fluorescence spectra automatcally generated

  • Spectral and kinetic analysis tools for both absorbance and fluorescence data

  • Methods and batch protocols for automating multiple sample measurement

'How Fast is Fast?'

Click here for the Aqualog 3.6.11 Patch.
Please Note: Please select to save the file. Release Notes.

 

NEW A-TEEM technology

A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!

All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.

Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.

 

NEW Aqualog® Datastream Dashboard

Features

  • Seamless integration with Aqualog
  • Convenient HTML-based interface
  • Push-button method operation
  • Simple administrator level controls for calibration and method development

Benefits

  •  Easy access through internet or intranet
  •  Dashboard shows the latest readings, time series and tables for trends and analysis
  •  WTP can upload their own independent data

Eigenvector Research, Incorporated Aqualog Datastream Dashboard is powered by Solo Predictor software from
Eigenvector Research, Incorporated

 

 

NEW Automatic Sipper Accessory

New for our Aqualog® A-TEEMTM spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.

The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection.  It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.
When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources.


Manufactured by HORIBA

Specifications

Aqualog Specification

Datastream Dashboard Specification

Automatic Sipper Accessory Specification

 

Aqualog Specification

Fluorescence Hardware

Choice of Light source

Standard: 150W ozone-free vertically mounted xenon arc lamp

Extended-UV: 150W vertically mounted xenon arc lamp

Excitation range

230 nm to upper limit of emission detector

200 nm to upper limit of emission detector

Excitation bandpass

5 nm

Excitation monochromator

Subtractive double monochromator

Excitation gratings

1200 gr/mm, 250 nm blaze

Excitation wavelength accuracy

±1 nm

Choice of Detector

UV-Visable

Red-extended

Emission range

250-620 nm

250-800 nm

Emission grating

405 gr/mm; 250 nm blaze

285 gr/mm; 350 nm blaze

Hardware pixel binning

0.41, 0.82, 1.64, 3.28 nm/pixel

0.58, 1.16, 2.32, 4.64 nm/pixel

Emission bandpass

5 nm

Emission spectrograph

Fixed, aberration-corrected 140 mm focal length

Emission detector

TE-cooled back-illuminated CCD

Emission integration time

5 ms minimum

 

CCD gain options

2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain

Sensitivity

Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)

Weight

32.72 kg (72 lbs)

Dimensions

LWH (618 x 435 x 336 mm); (24" x 17" x 13")

Absorbance Hardware

Scanning range

200 - 800 nm (UV lamp)
230-800 nm (Standard lamp)

Bandpass

5 nm

Slew speed

Max 500 nm/s

Optical system

Corrected single-beam

Detector

Si photodiode

Wavelength accuracy

±1 nm

Wavelength repeatability

+/- 0.5 nm

Photometric accuracy

±0.01 AU from 0 to 2A

Photometric stability

<0.002 AU per h

Photometric repeatability

 +/- 0.002 AU (0 to 1 AU)

Stray light

 

<1% measured with KI standard

Datastream Dashboard Specification

 

Aqualog Datastream Parameters

Specifications

Notes

Dissolved Organic Carbon Concentration
(DOC)

30 µg/l-20 mg/l

Requires Filtration (0.45 µm)

A254 nm

1 cm path length

SUVA

L  DOC mg-1
 A254 m-1

Simulated Distribution, System Trihalomethane,
Formation Potential

SDS THMFP (10 µg/l – 500 µg/l)

SDS THMFP (10 µg/l – 500 µg/l)

Parallel Factor Analysis Component Scores

Up to 7

Can include Algal, Oil/PAH, Tracer Dyes and other Components

Residuals (Q)

Detect Contaminants and Measurement Issues

% Variance Accounted For

Detect Contaminants and Measurement Issues

Absorbance Spectrum

200-800 nm; 1 cm path length

Any wavelength coordinate or ratio can be analyzed with extinction coefficients

Excitation Emission Matrix (EEM) Regions

I-V plus Algal (Bluegreen/Brown/Green)

Custom EEM regions also available; Based on Interpolated EEM
Processing

Total Fluorescence

Sum of EEM Regions I-V

Humic Index

Fluorescence Index

Independent Treatment Plant Data

DOC

mg/l

Alkalinity

mg/l

Chlorine Residual

mg/l

THM/SDSTHMFP

µg/l

A254 nm

Adjustable Path Length Specifications

pH

Additional Parameters Available

System Requirements

Datastream Specifications

Windows OS

64 bit Windows 7 or higher

Web Browser

Google Chrome ≥V53.x or Firefox  ≥49.x

Memory (RAM)

≥ 4 GB

Hard Disk

≥ 4 GB

DVD-ROM

Required

USB Ports

≥ 2

Additional ports may be required for accessories

Video Resolution

≥ 1024 x 768

Eigenvector Solo Industrial

V8.2 or higher

HORIBA Onsite Installation/Calibration

Required

HORIBA 6 month service

Advised

License Period

1 year renewable

Aqualog Hardware

UV-800 (200-800 nm)

Eigenvector Research, Incorporated Aqualog Datastream Dashboard is powered by Solo Predictor software from
Eigenvector Research, Incorporated

 

 

Automatic Sipper Accessory and Sample Changer Specifications

 

WS-10
Automatic Sipper Unit for Aqualog

WS-10-S
Sample Changer Unit for WS-10

Sample supply method

Suction using tubing pump

Suction from WS-10 Sipper

Amount of sample required

Up to 120mL/min +/-20mL/min at 25° C

Compliant tubing

Plastic tube, 2 mm inside, 3 mm outside

Maximum sampling
distance

Under 5 m from sampling point to auto sampler
(without sample changer)

Under 4 m from sampling point to sample
changer (under 1 m from sample changer to auto
sampler)

Sample water
requirements

Pressure: Under 0.20 MPa

SS: Particle size: 1.0 μm

External liquid leakage
sensor input

Operation resistance: range 3: 0 MΩ to 50 MΩ
Connecting cable: under 2 m

N/A

Connection

USB cable to computer

D-sub 25 pin to WS-10 sipper

Ambient temperature

5° C to 40° C

Relative humidity

20% to 85% (without dew condensation)

Storage temperature

0° C to 40° C (without freezing)

Power supply

100 V to 240 V AC +/-10% 50/60 Hz

From D-sub 25 pin connector

Structure

Indoor use

Size (W x D x H)

250 mm x 250 mm x 308 mm
9.84” x 9.84” x 12.13”

250 mm x 125 mm x 308 mm
9.84” x 4.92” x 12.13”

Weight

9 kg / 19.8 lbs.

4.2 kg / 9.26 lbs.

 

 

Accessories

NEW Automatic Sipper Accessory


New for our Aqualog® A-TEEM™ spectrometer, the Aqualog automatic sipper accessory handles sampling from a single source, in addition to dispensing rinsing solutions, detergents and controlling reverse-flow drainage.  The 4-sample changer unitconnects to the main sipper unit to enable sampling of up to 4 sources.

The sipper offers convenient installation and operation, with built-in automatic cleaning, leak detection and protection.  It is fully integrated into the new Aqualog 4.0 software for batch analysis and has a variety of uses in water, pharmaceutical, beverage phenolics and many other applications.

When used at a water treatment plant, the sipper and four channel accessory enable the Aqualog to automatically extract and monitor raw, settled and finished water samples. Each sample changer unit is compatible with overflow and filtration devices, serving up to 4 independent water treatment plant sources.

Download the Automatic Sipper Accessory flyer for more information.


Fluorescence webinar
OpenFluor Webinar

Videos