3D Raman confocal volume imaging provides detailed chemical imaging of a sample’s internal features – without the need for labelling or other preparation.  HORIBA Scientific’s true confocal Raman microscopes offer the best possible spatial resolution, ensuring minimum contribution from substrates and out-of-focus regions so that the resulting 3D volume images are clear, detailed and sharp.

 

Animation of a 3D volume map of a geological fluid inclusion
Animation of a 3D volume map of a geological fluid inclusion, showing the quartz matrix, aqueous carbon dioxide, and gaseous carbon dioxide.

3D Raman volume imaging typically acquires a Z-stack of XY images; the ensuing data cube is reconstructed into full 3D volume display using the integrated 3D Surface and Volume Display module within the LabSpec 6 spectroscopy software suite. Each voxel of the image contains a full Raman spectrum, which provides information on chemical composition, crystallinity, polymorphism and stress/strain.

Schematic showing the typical data structure of a 3D volume map (left) and its final rendering as a 3D volume (right)
Schematic showing the typical data structure of a 3D volume map (left) and its final rendering as a 3D volume (right)
Expanded polymer beads in a polymer matrix, with layer of immersion oil
Expanded polymer beads in a polymer matrix, with layer of immersion oil. [Data courtesy: Neil Everall, Intertek Wilton, UK]
Solid inclusion in an olivine mineral matrix
Solid inclusion in an olivine mineral matrix, showing 3D distribution of sulfur, magnesite, sulfate and water. [Data courtesy: Nathalie Bolfan, OPGC, Université Blaise Pascal Clermont-Ferrand, France]