Reagents for label-free interaction analysis

Our SPRi reagents have been especially designed for our SPRi systems. They allow you to work with confidence and save time for faster results.

SPRi Student kits

The SPRi starting kits are used to get acquainted with multiplex SPR imaging. Choose from two biological models and learn how SPRi can save you time and money.

Product name

Description

Protein biochip kit

Ready-to-use SPRi-Biochips and necessary reagents to perform a antibody-protein interaction

DNA biochip kit

Ready-to-use SPRi-Biochips and necessary reagents to perform a DNA-DNA interaction

SPRi Running buffers

Spri running buffers

Depending on the ligand/analyte interaction study, the composition of the running buffer is expected to improve specific binding. Salted buffers like PBS or HEPES are recommended. Buffer composition, concentration or pH must be adjusted according to the biomolecules studied.

Product name Description
PBS 0.01 M PBS pH 7.4
137 mM NaCl
2.7 mM KCl
PBS-T 0.01 M PBS pH 7.4
137 mM NaCl
2.7 mM KCl
0.005% (v/v) Tween 20
PBS 10X 0.1 M PBS pH 7.4
1.37 M NaCl
0.027 M KCl
PBS-T 10X 0.1 M PBS pH 7.4
1.37 M NaCl
0.027 M KCl
0.05% (v/v) Tween 20
TBS 5X 0.05 M TBS pH 7.4
150 mM NaCl
TBS-T 5X 0.05 M TBS pH 7.4
150 mM NaCl
0.005% (v/v) Tween 20
HBS 0.01 M HEPES pH 7.4
150 mM NaCl
HBS-T 0.01 M HEPES pH 7.4
150 mM NaCl
0.005% (v/v) Tween 20
HBS-E 0.01 M HEPES pH 7.4
150 mM NaCl
3 mM EDTA
HBS-ET 0.01 M HEPES pH 7.4
150 mM NaCl
3 mM EDTA
0.005% (v/v) Tween 20
SPRi buffer kit 1 Contains 4 buffers without Tween
SPRi buffer kit 2 Contains 4 buffers with Tween

SPRi Calibration solution

The calibration solution helps correct for sensitivity dispersions on the sensor chip surface.

Product name Description
N-SUC 3 mg sucrose to reconstitute in 1 mL of running buffer

SPRi activation kits

The SPRi activation kits should be used for primary amine coupling chesmitries with COOH groups ready to be activated (SAM CH-HD and CH-LD and 3D chemistries)

Product name Description
Amine coupling kit 1 Necessary reagents for NHS activation of COOH groups for amine coupling. To be reconstituted in 1 mL of filtered and deionised water.
Chemistry activation using NHS is more stable but less reactive.
Amine coupling kit 2 Necessary reagents for Sulfo-NHS activation of COOH groups for amine coupling. To be reconstituted in 1 mL of filtered and deionised water.
Chemistry activation using Sulfo-NHS is more reactive but less stable

SPRi Blocking solutions

SPRi Blocking solutions

The SPRi blocking solutions are used for deactivating the remaining reactive groups of the surface chemistry for covalent coupling. They can also help reduce non-specific binding.

Product name Description
B-Glycine 10 mM Glycine in PBS
B-Ethanolamine 1M Ethanolamine-HCl pH 9
B-BSA 1% BSA in PBS-T

SPRi Regeneration solutions

SPRi Regeneration solutions

The SPRi regeneration solutions are used to remove the bound analyte and any other non-covalently bound material while keeping the ligand activated. Regeneration conditions (concentration, contact time, pH…) must be optimized for each new ligand-analyte interaction study.

Product name Description
Glycine-HCl 100 mM Glycine - HCl pH 2
SDS 10% SDS
NaOH 50 mM NaOH
HCl 100 mM HCl
NaCl 1 M NaCl
SPRi Regeneration scouting kit Contains 5 regeneration solutions,
for exploring regeneration conditions.

SPRi Cleaning solutions

SPRi Cleaning solutions

The SPRi cleaning solutions are used for the daily cleaning and monthly maintenance of the SPRi systems.

Product name Description
C-NaOH 100 mM NaOH (for the monthly maintenance)
C-HCl 100 mM HCl (for the monthly maintenance)
SDS 10% SDS (for the daily cleaning)
SPRi Cleaning kit Contains 4 cleaning solutions