Fluorescence Spectroscopy
See Us at
- Advanced Materials Characterization Workshop - June 6th-7th
- Biofinders Texas Medical Center Commons- June 12th
- FluoroFest - August 1st - 3rd
- Biofinders University of VA School of Medicine - August 7th
- Biofinders VA Tech Corporate Research Park - August 8th
- Biofinders Fralin Life Sciences Institute - August 9th
News
- Aqualog CDOM Fluorometer Wins Award as one of Best New Instruments in 2011...Read more
- HORIBA Scientific and NKT Photonics Introduce the World’s First Commercial Integrated Supercontinuum Powered Spectrofluorometers... Read more
- Aqualog®, the only simultaneous absorbance and fluorescence system, measures CDOM (colored dissolved organic matter) in water 100 times faster than previous fluorescence methods...Read more
- World's First 100MHz Picosecond Diode Source With USB Interface and Plug-and-Play Interchangeable Heads ...Read more
Aqualog® - Our compact, Benchtop Fluorometer for CDOM
Overview
Aqualog is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.
Features
Hardware
- The only true simultaneous absorbance-fluorescence system available
- TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer
- Corrected UV-VIS absorbance detection path for stability and accuracy
- Double grating excitation monochromator for superior stray light rejection
- Matching bandpass for absorbance and fluorescence spectra
- Automatic sample changer option (2 or 4 position)
- Compatible with flow cells and titrator
Software
- Optimized experiment set-up menus minimize user confi guration time
- Complete NIST-traceable corrected fluorescence spectra automatcally generated
- Spectral and kinetic analysis tools for both absorbance and fluorescence data
- Methods and batch protocols for automating multiple sample measurement
Manufactured by HORIBA
Specifications
Fluorescence Hardware | |
|---|---|
Light source | 150W ozone free vertically mounted xenon arc lamp |
Excitation range | 240-630 nm |
Excitation bandpass | 5 nm |
Excitation monochromator | Subtractive double monochromator |
Excitation gratings | 1200 gr/mm, 250 nm blaze |
Excitation wavelength accuracy | ±1 nm |
Emission range | 210-620 nm |
Emission bandpass | 5 nm |
Emission spectrograph | Fixed, aberration-corrected 140 mm focal length |
Emission grating | 405 gr/mm; 250 nm blaze |
Emission detector | TE-cooled back-illuminated CCD |
Emission integration time | 5 ms minimum |
Hardware pixel binning | 0.41, 0.82, 1.64, 3.28 nm/pixel |
CCD gain options | 2.25 e-/cts in high gain, 4.5 e-/cts in medium gain, 9 e-/cts in low gain |
Sensitivity | Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration) |
Weight | 32.72 kg (72 lbs) |
Dimensions | LWH (618 x 435 x 336 mm); (24" x 17" x 13") |
Absorbance Hardware | |
|---|---|
Scanning range | 230 - 800 nm (optical) |
Bandpass | 5 nm |
Slew speed | Max 500 nm/s |
Optical system | Corrected single beam |
Detector | Si photodiode |
Wavelength accuracy | ±1 nm |
Wavelength repeatability | +/- 0.5 nm |
Photometric accuracy | ±0.01 AU from 0 to 2A |
Photometric stability | <0.002 AU per h |
Photometric repeatability | +/- 0.002 AU (0 to 1 AU) |
Stray light | <1% measured with KI standard |
Schematics
