FRET, or Forster Resonance Energy Transfer or Fluorescence Resonance Energy Transfer, is a phenomenon where closely matched pairs of fluorophores are used to determine spatial or temporal proximity and specificity in molecular, protein-protein interactions. More specifically, this energy transfer occurs when the emission energy of one fluorophore—the donor—is non-radiatively transferred to the second fluorophore—the acceptor—producing a secondary emission. When this occurs, donor fluorescence is quenched and acceptor fluorescence increases.

FRET Filter Sets (Forster Resonance Energy Transfer Filter sets or Fluorescence Resonance Energy Transfer Filter sets)

The Fret filter sets (Fluorescence Resonance Energy Transfer Filter sets) products listed below include the most commonly used FRET fluorophore pairs, as well as those recently developed pairs that are worthy of attention. These sets are comprised of the exciter and dichroic for the donor fluorophore and emitters for both the donor and acceptor fluorophores.

Individual filter set part numbers for both donor and acceptor fluorophores are also listed so components can be purchased individually, dependent on the specifics of the hardware set-up. If purchasing filters individually, it is important to remember that the exciter and dichroic from the acceptor fluorophore set are never used.

It is important that you provide hardware details and related mounting instructions when ordering filters for FRET applications.