Can Raman and epifluorescence measurements be made on the same instrument?

Yes, it is possible to combine Raman and epifluorescence on a single microscope system.

Epifluorescence imaging is widely used within biological fields for visualisation of cell/tissue materials, but does not offer the detailed molecular information that Raman can provide. Combining the two techniques on a single microscope system allows fast location of regions of interest within biological samples and targeted chemical analysis.  Experiments such as FISH (fluorescence in situ hybridisation) are routinely possible on such systems, allowing them to be combined with Raman chemical analysis.

Optical (left) and epifluorescence (right) images of single bacteria cells. The fluorescence is produced by FITC (green), Cy3 (red) and Cy5 (blue) fluorescence dyes.
Optical (left) and epifluorescence (right) images of single bacteria cells. The fluorescence is produced by FITC (green), Cy3 (red) and Cy5 (blue) fluorescence dyes. Data courtesy of Prof. Andrew Whiteley (Centre for Ecology and Hydrology, oxford, UK) and Dr Wei Huang (University of Sheffield, UK).
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