Surface Plasmon Resonance imaging (SPRi)
SPRi-PlexII™
Overview
SPRi-PlexII™ is the ideal solution for label-free and multiplexed bio-assays and investigations based on Surface Plasmon Resonance imaging technology (SPRi). This automated and compact apparatus is designed for simple operation and easy determination of real-time physico-chemical interactions and kinetics.
The SPRi-PlexII™ system provides automated monitoring of up to 400 biomolecular interactions occurring upon the functionalized surface of the SPRi Biochip™. The interaction between the ligands and the analytes can be studied rapidly and reliably with minimal user intervention.
Features
Automated SPR imaging system for assay measurements with:
- Multiplex Detection of several hundred interactions
- Label-Free protein, peptide, DNA samples
- Real-Time monitoring of kinetic curves
- Difference Image Display giving you a direct view of reactions as the experiment unfolds
- Fast Total Assay Time - typical assays in less than 10 minutes
- Temperature control
- Direct subtraction of the negative control
Manufactured by HORIBA Scientific
Specifications
- Sample volume: typically 200 μL
- Sample concentration: 150 ng/mL (100-1000 kDa) to 5 μg/mL (4-20 kDa)
- Sample molecular weight: > 240 Da
- Limit of detection: 5 pg/mm2
Accessories
| Continuous Flow Microspotter™(CFM) The CFM is an automatic printer that uses flow deposition for printing biomolecules. Samples are cycled over the surface and captured from solution, leading to higher biomolecule density, better spot uniformity and improved assay sensitivity. |
| SPRi-Arrayer SPRi-Arrayer is an automatic, compact and portable apparatus. It prints DNA, oligonucleotides, proteins, bacterial clones and other materials with low cross contamination. |
| Autosampler The Alias™ autosampler extends the automation potential of the SPRi-PlexII™ instrument and enables hundreds of unattended injections to be performed – saving you both time and money. |
