Carbaryl
Overview
Carbaryl is a carbamate insecticide. It is used for multiple farm products because of its long-lasting effect. This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of carbaryl that remains in farm products. This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretretreatment techniques, and sophisticated instrument operation as well as large quantities of acetone, methanol, hexane or acetic ether as organic solvents. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing carbaryl. 
Application examples
The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of carbaryl –added farm products (diluted to 10% methanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace carbaryl without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Pesticide | Cross-reactivity (%) |
|---|---|
Carbaryl | 100 |
Bendiocarb | 26.1 |
Benfuracarb | 2.8 |
Fentoate | 2.8 |
Isoprocarb | 2.1 |
Fenobcarb | 0.8 |
Fenitrothion | < 0.1 |
Acephate | < 0.1 |
Malathion | < 0.1 |
Fluvalinate | < 0.1 |
Cycloprothrin | < 0.1 |
Iprobenfos | < 0.1 |
Within-run reproducibility (spinach)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 22.8 | 1.6 | 6.9 |
B | 16.7 | 1.6 | 9.5 |
C | 3.6 | 0.4 | 11.4 |
n=8
Between-analysts reproducibility (spinach)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 20.9 | 1.7 | 8.0 |
B | 14.3 | 2.4 | 17.1 |
C | 2.2 | 0.6 | 24.8 |
n=3
Daily reproducibility (spinach)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 21.9 | 1.3 | 5.7 |
B | 14.3 | 2.3 | 16.1 |
C | 2.6 | 1.0 | 36.4 |
n=3
Recovery test (spinach)
Concentration of addition (ppm) | Recovery ratio (%) |
|---|---|
0.75 | 142.5±7.4 |
0.50 | 144.3±22.8 |
0.15 | 124.9±23.3 |
n=3
Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this test kit can quantitatively analyze carbaryl in produce.although the values fluctuate around the lower limit.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 12 columns | 1 piece |
Standard reagent L (freeze-dried: 1.5ppb) | 1mL (after dissolution) | 2 vials |
Standard reagent H (freeze-dried: 2ppb) | 1mL (after dissolution) | 2 vials |
Enzyme-labeled Carbaryl reagent (freeze-dried) | 6mL (after dissolution) | 2 vials |
Washing reagent (solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop reagent (solution) | 13mL | 1 vial |
Shelf-life | 12 months after production | Storage at 2 to 8°C |
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