Chlorothalonil

Overview

Chlorothalonil is an insecticide and is used for multiple farm products because of its effects on a wide range of mycotic diseases and its high residual efficacy.  This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of chlorothalonil that remains in farm products.  This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.

 

Features

          Comparison with instrumental analysis

While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as large quantities of acetone or hexane as organic solvents.  The simpler pretreatment required for kit saves significant time.  Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing chlorothalonil.

           Application examples

The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of chlorothalonil-added farm products (diluted to 10% methanol equivalent).  The overlapping curves; demonstrate the suitability of this kit for analyzing trace chlorothalonil without any interference from the original produce.


Manufactured by HORIBA

Specifications

The following data represent the basic kit performance.  For more detailed specifications, please contact us.

          Standard curve 

          Cross-reactivity

Pesticides

Cross-reactivity(%)

 

 

 

Chlorothalonil

100

Fthalide

39

PCP

1.7

Tecloftalam

< 0.1

Tetradifon

< 0.1

Cyazofamid

< 0.1

Cymoxanil

< 0.1

Benomyl

< 0.1

Procymidone

< 0.1

Iprodione

< 0.1

cis-Perumethorin

< 0.1

trans- Perumethorin

< 0.1

Oxin-Cu

< 0.1

Azoxystrobin

< 0.1

Metalaxyl

< 0.1

          Within-run reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

0.27

0.01

5.4

B

1.22

0.07

5.4

n=8

          Between-analysts reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

0.23

0.01

6.5

B

1.13

0.04

3.5

n=3

          Daily reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

0.25

0.01

5.6

B

1.17

0.04

3.6

n=3

          Recovery test (cucumber)

Concentration of addition (ppm)

Recovery ratio (%)

0.5

107.4±18.9

2.5

108.5±15.5

5.0

108.5±4.9

n=3
Results of within-run reproducibility, daily reproducibility, between-analysis reproducibility and recovery test show that this test kit can quantitatively analyze chlorothanonil in produce.

          Main kit components / shelf-life

Contents (form)

Volume

Quantity

Antibody coated plate (dry form)

8 row x 12 columns

1 piece

Standard reagent L (freeze-dried: 0.15ppb)

1mL (after dissolution)

2 vials

Standard reagent H (freeze-dried: 1.5ppb)

1mL (after dissolution)

2 vials

Enzyme-labeled Chlorothalonil reagent (freeze-dried)

6mL (after dissolution)

2 vials

Washing reagent (solution: 10 times concentrated)

50mL

1 vial

Chromogenic reagent (solution)

13mL

1 vial

Stop reagent (solution)

13mL

1 vial

Shelf-life:

12 months after production

Storage at 2 to 8°C