Chlothianidin
Overview
Chlothianidin is one of neonicotinoid insecticides. It is used for multiple farm products because of its wide insecticidal activity range. This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multiple sample analysis of chlothanidin that remains in farm products or in the environment. This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as large quantities of acetone, hexane or acetic ether as organic solvents. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing chlothianidin.
Application examples
The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of chlothianidin–added farm products (diluted to 10% methanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace Chlothianidin without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Neonicotinoid insecticides | Cross-reactivity (%) |
|---|---|
Chlothianidin | 100 |
Dinotefuran | 65 |
Acetamiprid | < 0.1 |
Imidacloprid | < 0.1 |
Thiacloprid | < 0.1 |
Nitenpyram | < 0.1 |
Thiamethoxam | < 0.1 |
Other insecticides and fungicides | Cross-reactivity (%) |
|---|---|
Nicotine sulfate | < 0.1 |
Pyrifenox | < 0.1 |
Carpropamid | < 0.1 |
Fthalide | < 0.1 |
Probenazole | < 0.1 |
Tebufenozide | < 0.1 |
Tricyclazole | < 0.1 |
Etofenprox | < 0.1 |
Comparison with instrumental analysis (brown rice)
Data courtesty of Dr. Eriko Hatakeyama, Research Institute for Environmental Science and Public Health of Iwate Prefecture
within-run reproducibility (cucumber)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 2.1 | 0.17 | 8.2 |
B | 4.9 | 0.30 | 6.0 |
C | 11.5 | 0.83 | 7.2 |
n=8
Between-analysts reproducibility (cucumber)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 2.3 | 0.03 | 1.1 |
B | 5.5 | 0.16 | 2.9 |
C | 12.6 | 0.35 | 2.8 |
n=3
Daily reproducibility (cucumber)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 2.0 | 0.29 | 14.3 |
B | 5.0 | 0.43 | 8.5 |
C | 11.6 | 0.56 | 4.8 |
n=3
Recovery test (cucumber)
Concentration of addition(ppm) | Recovery ratio (%) |
|---|---|
0.60 | 109.7±1.8 |
0.24 | 104.0±5.7 |
0.10 | 109.6±11.9 |
n=3
Results of within-run, daily reproducibility, between-analysis reproducibility and recovery test show that this kit can quantitatively analyze chlothianidin in produce.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 12 columns | 1 piece |
Standard reagent L (freeze-dried:1.5ppb) | 1mL(after dissolution) | 2 vials |
Standard reagent H (freeze-dried:15ppb) | 1mL(after dissolution) | 2 vials |
Enzyme-labeled Chlothianidin reagent (freeze-dried) | 6mL(after dissolution) | 2 vials |
Washing reagent(solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop reagent (solution) | 13mL | 1 vial |
Shelf-life: | 12 months after production | Storage at 2 to 8 °C |
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