Dinotefuran
Overview
Dinotefuran is one of neonicotinoid insecticides. It is used for multiple farm products because of its wide insecticidal activity range. This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of dinotefuran that remains in farm products or in the environment. This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as large quantity of acetonitrile as organic solvent. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing Dinotefuran. 
Application examples
The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of dinotefran-added farm products (diluted to 10% methanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace dinotefran without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Neonicotinoid insecticide | Cross-reactivity (%) |
|---|---|
Dinotefran | 100 |
Chlothianidin | 154 |
Acetamiprid | ＜0.1 |
Imidacloprid | ＜0.1 |
Thiacloprid | ＜0.1 |
Nitenpyram | ＜0.1 |
Thiamethoxam | < 0.1 |
Other insecticides and fungicides | Cross reactivity (%) |
|---|---|
Nicotine sulfate | ＜0.1 |
Ｐyrifenox | ＜0.1 |
Ｃarpropamid | ＜0.1 |
Ｆthalide | ＜0.1 |
Probenazole | ＜0.1 |
Tebufenozide | ＜0.1 |
Tricyclazole | < 0.1 |
Etofenprox | ＜0.1 |
Within-run reproducibility
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 2.1 | 0.17 | 8.2 |
B | 4.9 | 0.30 | 6.0 |
C | 11.5 | 0.83 | 7.2 |
n=8
Between-analysis (cucumber)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 2.3 | 0.35 | 15.3 |
B | 10.3 | 0.51 | 5.0 |
C | 30.8 | 0.83 | 2.7 |
n=3
Daily reproducibility (cucumber)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 2.4 | 0.10 | 4.3 |
B | 10.9 | 0.40 | 3.7 |
C | 32.7 | 1.04 | 3.2 |
n=3
Recovery test (cucumber)
Concentration of addition (ppm) | Recovery ratio (%) |
|---|---|
1.40 | 111.3±0.5 |
0.47 | 111.7±9.5 |
0.09 | 114.1±2.3 |
n=3
Results of within-run, daily reproducibility, between-analysis reproducibility and recovery test show that this kit can quantitatively analyze chlothianidin in produce.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 12 columns | 1 piece |
Standard reagent L (freeze-dried: 1.5ppb) | 1mL (after dissolution) | 2 vials |
Standard reagent H (freeze-dried: 30ppb) | 1mL (after dissolution) | 2 vials |
Enzyme-labeled Dinotefuran reagent (freeze-dried) | 6mL (after dissolution) | 2 vials |
Washing reagent (solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop reagent (solution) | 13mL | 1 vial |
Shelf-life: | 12 months after production | Storage at 2 to 8°C |
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