Emamectin
Overview
Emamectin is one of macrolide insecticides. It is used for multiple farm products because of its wide insecticidal activity range. This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multiple sample analysis of emamectin that remains in farm products or in the environment. This kit is best applied for inspedtion at delivery time/prior to processing or primary screening in production sites.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming fluorescent derivatization, and sophisticated instrument operation as well as a large quantity of organic solvent such as methanol. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing emamectin.
Application examples
The figures show the comparison between standard curve with 10% methanol and reaction curve drawn with methanol extract of emamectin-added farm products (diluted to 10% methanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace emamectin without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Structure analog | Cross-reactivity (%) |
|---|---|
Emamectin benzoate | 100 |
Emamectin metabolite (amino body) | 113 |
Emamectin metabolite (hormone amino body) | 55 |
Emamectin metabolite (N-methyl hormone amino body) | 38 |
Emamectin metabolite (8,9- Z isomeric form) | 9.1 |
Abamectin | 78 |
Milbemectin | < 0.1 |
Other natural origin pesticide | Cross-reactivity (%) |
|---|---|
Spinosyn A | < 0.1 |
Validamycin | < 0.1 |
Streptomycin | < 0.1 |
Within-run reproducibility (tomato)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 0.5 | 0.03 | 5.4 |
B | 1.4 | 0.07 | 5.0 |
C | 2.4 | 0.13 | 5.4 |
n=8
Between-analysts reproducibility (tomato)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 0.2 | 0.01 | 6.5 |
B | 0.6 | 0.03 | 4.7 |
C | 1.1 | 0.04 | 3.5 |
n=3
Daily reproducibility (tomato)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 0.5 | 0.03 | 5.6 |
B | 1.3 | 0.07 | 5.2 |
C | 2.4 | 0.09 | 0.09 |
n=3
Recovery test (tomato)
Concentration of addition (ppm) | Recovery ratio (%) |
|---|---|
0.30 | 103.9±2.9 |
0.10 | 99.8±4.2 |
0.03 | 108.9±3.6 |
n=3
Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this kit can quantitatively analyze emamectin in procuce.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 12 columns | 1 piece |
Standard reagent L (freeze-dried: 1.5ppb) | 2mL | 1 vial |
Standard reagent H (freeze-dried: 15ppb) | 2mL | 1 vial |
Enzyme-labeled Emamectin reagent (freeze-dried) | 6mL (after dissolution) | 2 vials |
Dissolution / dilution reagent | 40mL | 1 vial |
Washing reagent (solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop reagent (solution) | 13mL | 1 vial |
Shelf-life: | 12 months after production | Storage at 2 to 8°C |
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