Flutolanil

Overview

Flutolanil is a benzanilide fungicide that has pest control effect for rice sheath blight disease.  It is used for not only rice but also vegetables or fruits.  This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of flutoranil that remains in farm products.  This kit is best applied for inspection at delivery time/prior to processing inspection or primary screening in production sites.

 

Features

          Comparison with instrumental analysis

While instrument analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as large quantities of acetone, dichloromethane or hexane as organic solvents.  The simpler pretreatment required for kit saves significant time.  Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing flutolanil.

          Application examples 

 

The figures show the comparison between standard curves with 10% methanol and reaction curved drawn with methanol extract of flutolanil-added farm products (diluted to 10% mthanol equivalent).  The overlapping curves; demonstrate the suitability of this kit for analyzing trace flutolanil without any interference from the original produce.

 

Manufactured by HORIBA

Specifications

The following data represent the basic kit performance. For more detailed specifications, please contact us.

Standard curve 

 

Cross-reactivity

Pesticides

Cross-reactivity(%)

Flutolanil

100

Mepronil

30.1

Oxycarboxin

< 0.1

Furametpyr

< 0.1

Propiconaazole

< 0.1

Metalaxyl

< 0.1

Fthalide

< 0.1

Buprofezin

< 0.1

Etofenprox

< 0.1

Chlorpyrifos-methyl

< 0.1

Phenthoate

< 0.1

Fenitrothion

< 0.1

          Correlation with instrumental analysis (tomato)

Data courtesy of Dr. Koichi Shiraki, Gifu Prefectural Research Institute for Health and Environmental Sciences.

Within-run reproducibility (spinach)

Samples

Average (ppb)

SD

CV(%)

A

5.1

0.29

5.6

B

3.9

0.19

4.8

C

2.7

0.16

6.0

n=8

Between-analysts reproducibility (spinach)

Samples

Average (ppb)

SD

CV(%)

A

4.8

0.30

6.2

B

3.8

0.25

6.6

C

2.4

0.19

8.0

n=3

Daily reproducibility (spinach)

Samples

Average (ppb)

SD

CV(%)

A

5.1

0.06

1.2

B

4.1

0.22

5.4

C

2.7

0.13

4.9

n=3

Recovery test (spinach)

Concentration of addition (ppm)

Recovery ratio(%)

0.20

119.9±5.6

0.15

131.5±5.3

0.10

113.3±3.1

n=3

Results of with-in run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this test kit can quantitatively analyze ｆlutoranil.

          Main kit components / shelf-life

Contents (form)

Volume

Quantity

Antibody coated plate (dry form)

8 rows x 12 columns

1 piece

Standard reagent L (freeze-dried: 1ppb)

1mL (after dissolution)

2 vials

Standard reagent H (freeze-dried: 8ppb)

1mL (after dissolution)

2 vials

Enzyme-labeled Flutolanil reagent (freeze-dried)

6mL (after dissolution)

2 vials

Washing reagent (solution: 10 times concentrated)

50mL

1 vial

Chromogenic reagent (solution)

13mL

1 vial

Stop reagent (solution)

13mL

1 vial

Shelf-life:

10 months after production

Storage at 2 to 8°C