H-Fenitrothion

Overview

Fenitrothion is an organic phosphorous insecticide.  It is used for multiple farm products.  This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of fenitrothion that remains in farm products.  This kit is best applied for inspection af delivery time /prior to processing or primary screening in production sites.

 

 

Features

Comparison with instrumental analysis

While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as  large quantities of hexane or acetone as organic solvents.  The simpler pretreatment required for kit saves significant time.  Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing fenitrothion.

 

 Application examples

 

The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of fenitrothion-added farm products (diluted to 10% methanol equivalent).  The overlapping curves; demonstrate the suitability of this kit for analyzing trace fenitrothion without any interference from the original produce.  

Manufactured by HORIBA

Specifications

The following data represent the basic kit performance. For more detailed specifications, please contact us.  

Standard curve

Cross-reactivity

Pesticides

Cross-reactivity (%)

Fenitrothion

100

EPN

34

Parathion

12

Parathion-methyl

6.9

Fenitro oxon

1.0

Cyanophos

0.9

EPN Oxon

< 0.1

Fenthion

< 0.1

Diclofenthion

< 0.1

Diazinon

< 0.1

Isoxathion

< 0.1

Clorpirifos

< 0.1

Clorpirifos-methyl

< 0.1

Fenate

< 0.1

Chlorfenvinphos

< 0.1

Sulprofos

< 0.1

Protiophos

< 0.1

Propaphos

< 0.1

Profenophos

< 0.1

          Within-run reproducibility (brown rice)

Samples

Average (ppb)

SD

CV(%)

A

0.90

0.03

3.3

B

0.51

0.04

8.6

C

0.37

0.02

6.2

n=8

Between-analysts reproducibility (brown rice)

Samples

Average (ppb)

SD

CV(%)

A

0.85

0.03

4.0

B

0.50

0.02

4.9

C

0.37

0.05

12.3

n=3

          Daily reproducibility (brown rice)

Samples

Average (ppb)

SD

CV(%)

A

0.93

0.06

6.7

B

0.52

0.05

9.7

C

0.37

0.01

1.4

n=3

          Recovery test (brown rice)

Concentration of addition (ppm)

Recovery ratio (%)

0.030

151.0±0.8

0.020

149.2±14.6

0.015

138.1±4.5

n=3

Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery tests show that this test kit can quantitatively analyze fenitrothion in produce. 

Main kit components / shelf-life

Contents (form)

Volume

Quantity

Antibody coated plate (dry form)

8 rows x 12 columns

1 piece

Standard reagent L (freeze-dried: 0.2ppb)

1mL (after dissolution)

2 vials

Standard reagent H (freeze-dried: 2ppb)

1mL (after dissolution)

2 vials

Enzyme-labeled Fenitrothion reagent (freeze-dried)

6mL (after dissolution)

2 vials

Washing reagent (solution: 10 times concentrated)

50mL

1 vial

Chromogenic reagent (solution)

13mL

1 vial

Stop reagent (solution)

13mL

1 vial

Shelf-life:

12 months after production

Storage at 2 to 8°C