Imazalil
Overview
Imazalil is a widely used fungicide as post-harvest pesticide particularly for imported citrus, it is likely to remain in foods. This kit is best applied for the test prior to over-the-counter sales of citrus using imazalil. This kit monitors that pesticide residue is below the criterion value rapidly (within 2 hours) and quantitatively.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as large quantities of acetic ether, acetonitrile or acetone as organic solvents. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing imazalil.
Application examples
The figures show the comparison between standard curves with 10% methanol and reaction curved drawn with methanol extract of imazalile-added farm products (diluted to 10% mthanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace imazalile without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Pesticides | Cross-reactivity (%) |
|---|---|
Imazalil | 100 |
Penconazole | 0.3 |
Tetraconazole | < 0.1 |
Hexaconazole | < 0.1 |
Fenbuconazole | < 0.1 |
Myclobutanil | < 0.1 |
Tebuconazole | < 0.1 |
Triflumizole | < 0.1 |
Propiconaazole | < 0.1 |
Chiabendazole | < 0.1 |
Methidathion | < 0.1 |
OPP | < 0.1 |
Correlation with instrumental analysis (citrus)
Data courtesy of Dr. Eriko Hatakeyama, Research Institute for Environmental Science and Public Health of Iwate Prefecture.
Within-run reproducibility (lemon)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 31 | 2.6 | 3.6 |
B | 18 | 2.7 | 9.4 |
n=8
Between-analysts reproducibility (lemon)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 31 | 3.9 | 5 |
B | 18 | 2.9 | 10.8 |
n=3
Daily reproducibility (lemon)
Samples | Average (ppb) | SD | CV(%) |
|---|---|---|---|
A | 29 | 1.4 | 1.9 |
B | 17 | 3.4 | 12.5 |
n=3
Recovery test (lemon)
Concentration of addition (ppm) | Recovery ratio (%) |
|---|---|
1.25 | 107.0±10.1 |
0.75 | 99.8±18.5 |
0.51 | 96.4±15.1 |
n=3
Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this kit can quantitatively analyze imazalil in produce.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 13 columns | 1 piece |
Standard reagent L (freeze-dried: 5ppb) | 1mL (after dissolution) | 2 vials |
Standard reagent H (freeze-dried: 50ppb) | 1mL (after dissolution) | 2 vials |
Enzyme-labeled Imazalil reagent (freeze-dried) | 6mL (after dissolution) | 2 vials |
Washing reagent (solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop reagent (solution) | 13mL | 1 vial |
Shelf-life: | 12 months after production | Storage at 2 to 8°C |
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