Isoprothiolane
Overview
Isoprothiolane is one of fungicides for Blast disease and is also used as a dithiolane pesticide. It is used for cultivation of rice plant because of its effect to brown planthoppers. This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of isoprothiolane that remains in farm products. This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as, large quantities of hexane or acetic ether as organic solvents and a wide variety of solvents. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing Isoprothiolane.
Application examples
The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of isoprothiolane –added farm products (diluted to 10% methanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace isoprothiolane without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Pesticides | Cross-reactivity (%) |
|---|---|
Ｉsoprothiolane | 100 |
Diazinon | < 0.1 |
Propaphos | < 0.1 |
Fenitrothion | < 0.1 |
Buprofezin | < 0.1 |
Benfuracarb | < 0.1 |
Fipronil | < 0.1 |
Etofenprox | < 0.1 |
Flutlanil | < 0.1 |
Furametpyr | < 0.1 |
Probenazole | < 0.1 |
Iprobenfos | < 0.1 |
Fenobucarb | < 0.1 |
Thiophanate-methyl | < 0.1 |
Pyroquilon | < 0.1 |
Within-run reproducibility (brown rice)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 51.7 | 4.6 | 9.0 |
B | 25.0 | 2.5 | 10.1 |
n=8
Between-analysts reproducibility (brown rice)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 39.2 | 1.0 | 2.6 |
B | 70.6 | 6.7 | 9.5 |
n=3
Daily reproducibility (brown rice)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 46.3 | 4.8 | 10.4 |
B | 22.1 | 2.7 | 12.2 |
n=3
Recovery test (brown rice)
Concentration of addition (ppm) | Recovery ratio (%) |
|---|---|
2.0 | 110.5±6.0 |
1.0 | 116.7±10.4 |
0.5 | 102.1±6.8 |
n=3
Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this test kit can quantitatively analyze isoprothiolane in produce.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 12 columns | 1 peace |
Standard reagent L (freeze-dried: 6ppb) | 1mL (after dissolution) | 2 vials |
Standard reagent H (freeze-dried: 100ppb) | 1mL (after dissolution) | 2 vials |
Enzyme-labeled Isoprothiolane reagent (freeze-dried) | 6mL (after dissolution) | 2 vials |
Cleaning reagent (solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop reagent (solution) | 13mL | 1 vial |
Shelf-life: | 12 months after production | Storage at 2 to 8°C |
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