Myclobutanil

Overview

Myclobutanil is a Triazole fungicide and is used for multiple farm products.  This kit allows an easy, rapid (within 2 hours), sensitive, quantitative,multi-sample analysis of myclobutanil that remains in farm products.  This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.

 

Features

Comparison with instrumental analysis

While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as , and also a large quantity of acetonitrile, hexane, dicloromethane or Ethyl acetate as organic solvent.  The simpler pretreatment required for kit saves significant time.  Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing myclobutanil.

          Application examples

The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of myclobutanil-added farm products (diluted to 10% methanol equivalent).  The overlapping curves; demonstrate the suitability of this kit for analyzing trace myclobutanil without any interference from the original produce.


Manufactured by HORIBA

Specifications

The following data represent the basic kit performance. For more detailed specifications, please contact us.

Standard curve

          Cross-reactivity

Pesticides

Cross-reactivity (%)

Myclobutanil

100

Miclobutanil hydroxyl body

26.7

Hexaconaozole

< 0.1

Penconazole

< 0.1

Propiconazole

< 0.1

Triadimenol

< 0.1

Acetaprimid

< 0.1

Iprodione

< 0.1

Iminoctadine albesilate

< 0.1

Cypermethrin

< 0.1

Triadimefon

< 0.1

Triflumizole

< 0.1

Perumethorin

< 0.1

Malathion

< 0.1

Within-run reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

0.33

0.039

11.7

B

1.19

0.105

8.8

n=8

          Between-analysts reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

0.84

0.08

9.7

B

1.38

0.23

16.8

n=3

Daily reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

0.32

0.014

4.4

B

1.18

0.015

1.3

n=3

Recovery test (cucumber)

Concentration of addition (ppm)

Recovery ratio (%)

0.48

76.7±9.5

0.08

90.1±6.6

0.03

76.5±32.7

n=3

Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this test kit can quantitatively analyze myclobutanil in produce.

          Main kit components / shelf-life

Contents (form)

Volume

Quantity

Antibody coated plate (dry form)

8 rows x 12 columns

1 piece

Standard reagent L (freeze-dried: 0.2ppb)

1mL (after dissolution)

2 vials

Standard reagent H (freeze-dried: 2ppb)

1mL (after dissolution)

2 vials

Enzyme-labeled Myclobutanil eagent (freeze-dried)

6mL (after dissolution)

2 vials

Washing reagent (solution: 10 times concentrated)

50mL

1 vial

Chromogenic reagent (solution)

13mL

1 vial

Stop reagent (solution)

13mL

1 vial

Shelf-life:

8 months after production

Storage at 2 to 8°C