Nitenpyram

Overview

Nitenpyram is one of neonicotinoid insecticides.  It is used for multiple farm products because of its high osmosis transitivity.  This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of nitenpyram that remains in farm products or in the environment.  This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.

 

Features

          Comparison with instrumental analysis

While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as  large quantities of acetone, methanol or ammonia as organic solvents.  This simpler pretreatment required for kit saves significant time.  Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing nitenpyram.

          Application examples

The figures show the comparison between standard curves with 10% methanol and reaction curves drawn with methanol extract of nitenpyram-added farm products (diluted to 10% methanol equivalent).  The overlapping curves; demonstrate the suitability of this kit for analyzing trace nitenpyram without any interference from the original produce.

Manufactured by HORIBA

Specifications

The following data represent the basic kit performance.  For more detailed specifications, please contact us. 

          Standard curve 

Cross-reactivity

Pesticide

Cross-activity (%)

Nitenpyram

100

CPMA 

1.6

CPMF 

< 0.1

Acetamiprid

< 0.1

Imidacloprid

< 0.1

Thiacloprid

< 0.1

Thiamethoxam

< 0.1

Dinotefuran

< 0.1

Nicotine sulfate

< 0.1

Pyrifenox

< 0.1

Validamycin A

< 0.1

Fthalide

< 0.1

(Z)-Ferimzone

< 0.1

Cartap

< 0.1

          Within-run reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

5.6

0.15

2.7

B

20

0.69

3.5

C

100

1.6

1.6

n=8

          Between-analysts reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

5.9

0.15

2.5

B

21

1.8

8.5

C

100

3.4

3.3

n=3

          Daily reproducibility (cucumber)

Samples

Average (ppb)

SD

CV(%)

A

5.7

0.12

2.1

B

20

1.3

6.2

C

100

0.80

0.8

n=3

          Recovery test (cucumber)

Concentration of addition (ppm)

Recovery ratio (%)

5.0

105±2.3

1.0

109±1.9

0.3

107±1.5

n=3

Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this test kit can quantitatively analyze iprodione in procuce.

Main kit components / shelf-life

Contents (form)

Volume

Quantity

Antibody coated plate (dry form)

8 rows x 12 columns

1 piece

Standard reagent L (freeze-dried: 5ppb)

1mL (after dissolution)

2 vials

Standard reagent H (freeze-dried: 100ppb)

1mL (after dissolution)

2 vials

Enzyme-labeled Nitenpyram reagent (freeze-dried)

6mL (dissolution)

2 vials

Washing reagent (solution: 10 times concentrated)

50mL

1 vial

Chromogenic reagent (solution)

13mL

1 vial

Stop reagent (solution)

13mL

1 vial

Shelf-life:

12 months after production

Storage at 2 to 8°C