Triflumizole
Overview
Triflumizole is an imidazole fungicide and is used for multiple farm products. This kit allows an easy, rapid (within 2 hours), sensitive, quantitative, multi-sample analysis of triflumizole that remains in farm products. This kit is best applied for inspection at delivery time/prior to processing or primary screening in production sites.
Features
Comparison with instrumental analysis
While instrumental analysis offers high accuracy, it requires complicated and time-consuming pretreatment techniques, and sophisticated instrument operation as well as large quantities of acetone, acetic acid, acetonitrile or hexane as organic solvents. The simpler pretreatment required for kit saves significant time. Additionally, only a small quantity of methanol is required as an extraction solvent for analyzing triflumizole.
Application examples
The figures show the comparison between standard curves with 10% methanol and reaction curve drawn with methanol extract of triflumizole-added farm products (diluted to 10% methanol equivalent). The overlapping curves; demonstrate the suitability of this kit for analyzing trace triflumizole without any interference from the original produce.
Manufactured by HORIBA
Specifications
The following data represent the basic kit performance. For more detailed specifications, please contact us.
Standard curve
Cross-reactivity
Pesticides | Cross-reactivity (%) |
|---|---|
Triflumizole | 100 |
Triflujizole metabolite | 3.7 |
Triadimefon | < 0.1 |
Bitertanol | < 0.1 |
Myclobutanil | 0.1 |
Hexaconazole | < 0.1 |
Imazalil | 0.2 |
Imibenconazole | 0.2 |
Prochloraz | 2.8 |
Correlation with instrument analysis (cucumber)
Data courtesy of Mr. Toshiyoshi Nakata, Hiyoshi Co., Ltd.
Within-run reproducibility (grape tomato)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 3.4 | 0.22 | 6.4 |
B | 8.3 | 0.61 | 7.3 |
C | 15.8 | 0.50 | 3.2 |
n=8
Between-analysts reproducibility (grape tomato)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 3.4 | 0.51 | 14.9 |
B | 9.6 | 0.58 | 5.9 |
C | 16.8 | 0.84 | 4.7 |
n=3
Daily reproducibility (grape tomato)
Samples | Average(ppb) | SD | CV(%) |
|---|---|---|---|
A | 3.9 | 0.7 | 18.6 |
B | 9.4 | 1.1 | 11.7 |
C | 17.3 | 2.3 | 13.3 |
n=3
Recovery test (grape tomato)
Concentration of addition (ppm) | Recovery ratio(%) |
|---|---|
3.0 | 112.9±5.0 |
1.6 | 109.5±2.6 |
0.6 | 125.0±2.3 |
n=3
Results of within-run reproducibility, daily reproducibility, between-analysts reproducibility and recovery test show that this test kit can quantitatively analyze triflumizole in produce.
Main kit components / shelf-life
Contents (form) | Volume | Quantity |
|---|---|---|
Antibody coated plate (dry form) | 8 rows x 12 columns | 1 piece |
Standard reagent L (freeze-dried: 2ppb) | 1mL (after dissolution) | 6 vials |
Standard reagent H (freeze-dried: 20ppb) | 1mL (after dissolution) | 6 vials |
Enzyme-labeled Triflumizole reagent (freeze-dried) | 6mL (after dissolution) | 2 vials |
Washing reagent (solution: 10 times concentrated) | 50mL | 1 vial |
Chromogenic reagent (solution) | 13mL | 1 vial |
Stop eagent (solution) | 13mL | 1 vial |
Shelf-life: | 12 months after production | Storage at 2 to 8°C |
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