Homogenization of Milk

Milk is a very complex food containing over 100,000 different molecular species. Cow’s milk is composed of 87% water, 3.5% protein, 3.7% fat, 4.9% lactose, and 0.7% salts. The main components of interest are protein and fat globules. The proteins, referred to as casein particles combine together with calcium and phosphate to form aggregates with a well-defined structure. Their typical size is in the range of 100 nm. They are white, tasteless and odorless and are used to make food, paint, and adhesives. In contrast, the fat globules in raw unhomogenized milk are between 1-10 µm, while in homogenized milk the size range is 0.2-2 µm.

Typical milk factory

Homogenization Process

Milk is an oil-in-water emulsion, with the fat globules dispersed in a continuous phase of skim milk. If raw milk were left to stand the fat globules would form a cream layer and rise to the surface.

Homogenization is a mechanical process used to reduce the size of the fat globules in the milk. The net result of this process is a decreased creaming rate according to Stokes’ Law, reduced clustering during creaming, and better density matching with the continuous phase.

The homogenization process reduces the globule size by passing milk under high pressure through a tiny orifice. To understand the mechanism, consider a conventional homogenizing valve (Figure 1) processing an emulsion such as milk at a flow rate of 20,000 l/hr. As it first enters the valve, liquid velocity is about 4 to 6 m/s. It then moves into the gap between the valve and the valve seat and its velocity increases to 120 meters/sec in about 0.2 milliseconds. The liquid then moves across the face of the valve seat and exits in about 50 microseconds. The homogenization phenomenon is completed before the fluid leaves the area between the valve and the seat.

Schematic image of homogenization valve.
Figure 1

While most of the fat globule reduction takes place in the first stage, there is a tendency for clumping or clustering of the reduced fat globules. The second stage valve shown in Figure 1 permits the separation of those clusters into individual fat globules, as seen in Figure 2 below. The second stage is similar to the first stage, as seen in the diagram above. The two-stage homogenizer permits precise control of the homogenization process. Different valve setting give different size emulsions, which in turn affect the final products’ stability, taste, and shelf life.

Effect of two stage homogenization on particle size

Process Monitoring and Control

Horiba’s LA-300 has proven to be a popular analyzer for monitoring this process because of its low cost and ease of operation. The analyzer can be placed at each milk plant to provide real-time feedback on the homogenization process. This allows for optimization of the final product, cost savings by eliminating overprocessing of the material and out-of spec material, and evaluation of the homogenizer itself for wear or breakage.

Raw Milk Size Analysis

The distribution below is the result of measurement of whole raw, (unhomogenized milk) with the LA-300 particle size analyzer. The distribution shows the large fat globules prior to homogenization. The ratio and size of fat globules to casein is such that the caseins are not seen in whole milk.

Particle Size of Raw Milk

Whole Milk Size Analysis

This distribution is of whole homogenized milk. This is a stable distribution of the milk emulsion. The largest globules are reduced from 15 µm to 3 µm. The LA-300 monitors this size reduction very well.

Particle Size of Whole Milk

2 % Milk Size Analysis

In the 2% milk, where a large portion of the fat has been removed, the main peak stays centered around one micron while a slight shoulder appears on the small side. This represents the caseins which now show up in the distribution, due to the removal of the fat globules.

Particle Size of 2 % Milk

1 % Milk Size Analysis

In the case of 1 % milk even more fat is removed and so the ratio of emulsified fat globules, to the protein caseins, is much lower. This results in the protein appearing as a second mode.

Particle Size of 1 % Milk

Nonfat Milk Size Analysis

Since non-fat milk lacks the emulsified fat globules the distribution is made up primarily of the protein caseins. The remaining distribution is just the proteins.

Particle Size of Nonfat Milk

Concluding Comments

The main goal of homogenization is to break up the large fat globules and create a stable emulsion that has an increased shelf life, a better taste, and improved mouth feel. The LA-300 is an excellent tool to monitor this process. It is able to show the large end of the distribution shift from 10 µm to about 2 µm without a problem. Also, in the low fat milk it is able to see the size distribution of the proteins.

For those customers who have a larger capital budget, the LA-960 would provide even more data at the smaller size ranges where the proteins are seen in addition to monitoring the reduction in fat globule size in the large size range.

Analyzers for Milk Particle Size


The LA-960V2 uses Mie Scattering (laser diffraction) to measure particle size of suspensions or dry powders. The speed and ease-of-use of this technique makes it the popular choice for most applications.