Key Features and Performance Benefits

• 2-in-1 Simultaneous Fluorescence and Absorbance Spectrometer
• UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm
• Millisecond CCD Detection of Entire Fluorescence Spectrum
• Full 3-D Fluorescence EEM Acquisition in Less Than One Second
• Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman
• Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)
• High Fidelity Molecular Fingerprinting with Unique A-TEEM™ (Absorbance-Transmittance Excitation Emission Matrix) Technology

Applications Powered by Duetta

• Protein Aggregation, Amyloid Diseases and Drug Development
• Biomarker and Probe Development
• Nanomedicine & Phototherapy

• Pollutant Detection & Water Treatment
• Photocatalysis

“We endeavored to take as much advantage as possible of the wide excitation and emission capability of the HORIBA instrument (excitation/ emission matrix, EEM) so that emission at unexpected wavelengths was not missed.”
- S. Sma-Air et al, Spectrofluorometric insights into the application of PAM fluorometry in photosynthetic research

• Algal and Chlorophyll Analysis

• Quantum Dots & Nanomaterials

"EEM spectroscopy can be implemented as a powerful technique for determining the purity of complex mixtures, especially when other techniques, including mass spectrometry, fail to provide adequate characterization of a given material."... "The determination of different cluster species was only made possible with the complete fluorescence profiles provided from EEM spectroscopy."
- K. Stamplecoskie et al. "The power of fluorescence excitation–emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures of fluorescent silver clusters"

"The inherent value of EEMS is not just for characterizing a single emission species!”
- H. Ramsay et al. "The power of fluorescence excitation-emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures fluorescent silver clusters.”

"EEM provided a thorough evaluation of the correlations between the excitation and emission spectra."
- S. Takahama et al, “Variation in the responses of carbon quantum dots (CQDs) synthesized from native coconut husk and coconut husk-derived charcoal.”

• Phosphorescence and Fluorescent Materials

“Using EEM spectroscopy, it is possible to visualize the complete fluorescence profile of each of the three cluster species isolated from RP-HPLC."
- H. Ramsey et al, "The power of fluorescence excitation-emission matrix (EEM) spectroscopy in the identification and characterization of complex mixtures fluorescent silver clusters."

• Thin Films & Hybrid Materials

• Chemo - and Biosensors

"The Duetta can measure absorbance and fluorescence simultaneously allowing easy inner filter correction. All spectra were corrected for both Rayleigh scattering and inner filter effects."
- AW Harrison et al, “Reaction of Glyoxal and Ammonium as a Potential Contributor to Protein-like Fluorescence in Atmospheric Measurements.”

• Molecular Probes & Logic Gates

• Oil Recovery
• Solar Cells

• Contaminant Detection
• Protein and Nutrient Analysis

"EEM was an efficient method for determining the optical properties of CQDs in detail." 
-    S. Takahama et al, “Variation in the responses of carbon quantum dots (CQDs) synthesized from native coconut husk and coconut husk-derived charcoal.”

Why Scientists Choose Duetta 

• Speed & Sensitivity: Capture full spectra in milliseconds, enabling kinetic studies and high-throughput screening. 
• Broad Spectral Range: Analyze samples from UV to NIR, including challenging nanomaterials and biological tissues. 
• Advanced Data Analysis: Integrated multivariate tools and A-TEEM™ technology for robust molecular fingerprinting and correction of inner-filter effects. 
• Versatility: Seamlessly switch between absorbance and fluorescence modes for comprehensive sample characterization.

Featured Video

A Superior Spectrometer Concept

The Future of Fluorescence

• Three-in-one Fluorescence and Absorbance Spectrometer
• UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm
• Full 3-D Fluorescence EEM Acquisition in Less Than One Second
• Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman
• Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)
• High Fidelity Molecular Fingerprinting with Unique A-TEEM™ (Absorbance-Transmittance Excitation Emission Matrix) Technology
• Millisecond CCD Detection of Entire Fluorescence Spectrum

Fluorescence and Absorbance in one Spectrometer!

Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.

Fluorescence in the Blink of an Eye!

Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in the world, and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.

Highest Fluorescence Sensitivity in its Class

Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations of a sample, and provides better quality data.

EzSpec™ Touch-Screen Software!

Duetta debuts HORIBA’s new generation software interface with touch-screen utility, and a selection of Apps for routine analysis.