Summary: A-TEEM (Absorbance-Transmittance Excitation-Emission Matrix) is a multi-dimensional analytical technique that identifies cell culture media variability that Raman, LC-MS, and 2D-Fluorescence miss. By providing a quantitative 'molecular fingerprint' in seconds, A-TEEM prevents batch failures, ensures GMP regulatory compliance, and reduces QC costs.
- Speed: Results in seconds, not hours (vs. LC-MS).
- Sensitivity: Detects trace contaminants invisible to 2D Fluorescence.
- Simplicity: No solvents, minimal sample prep, PAT-ready.
A-TEEM (Absorbance, Transmittance, and Excitation-Emission-Matrix) spectroscopy, combined with multivariate analysis, is a rapid, non-invasive, and cost-effective technique for comprehensively characterizing cell culture media, providing molecular fingerprints to monitor lot-to-lot variability and detect subtle compositional changes that directly impact cell growth and final product quality.
BioPharm International recently published an article on this topic, titled 'A-TEEM: A Novel, Powerful Tool for Evaluating and Monitoring Cell Culture Media'. The publication highlights how A-TEEM, by enabling the simultaneous optical characterization of media, offers molecular-level insights into composition, thereby enhancing quality control and ensuring media consistency in biopharmaceutical manufacturing. Specifically, the article demonstrates the effectiveness of A-TEEM in assessing lot-to-lot variability and providing a robust, cost-effective approach for ensuring media quality before its use in bioreactors.
Read the full story on BioPharm International to explore the data and case studies.