Xiaohong Fang received her Ph.D. degree in Analytical Chemistry at Peking University (China) in 1996. After one-year postdoc work in Univ. of Waterloo (Canada), she worked as a research associate in Univ. of Florida (USA) from 1998 to 2001. She was recruited to the Chinese Academy of Sciences (CAS) in the “Hundred Talents Program” in December 2001, and then became a professor of chemistry in Key Laboratory of Molecular Nanostructures and Nanotechnology at Institute of Chemistry, CAS. She is also an adjunct professor of the University of the Chinese Academy of Sciences. Since 2007, she has been appointed as a chief scientist of the National Key Basic Research Program on Nanosciences by Ministry of Sciences and Technology of China.

Her main research interest is the development of new biophysical and bioanalytical methods for the real-time protein detection and for the study of biomolecular interaction at the single molecule level. This includes: single molecule imaging and monitoring in living cells, biomolecular interaction study by single-molecule force microscopy, novel molecular probes for real-time protein recognition and nanobiosenor development. She has over 170 publications in scientific journals such as PNASJACSAngew. Chem. Int. Ed.Adv. Mater., and over 20 granted patents.


Monitoring Membrane Receptor Dynamics in Living Cells by Single-Molecule Fluorescence Imaging

Single-molecule fluorescence imaging of receptor dynamics is becoming a powerful tool for the real-time study of receptor activation and internalization to achieve a better understanding of signal transduction mechanism. With single-molecule imaging of green fluorescent protein (GFP) tagged transforming growth factor β (TGFβ) receptors at the cell surface, we have proposed a new model in which the activation of serine-threonine kinase receptors is accomplished via monomer dimerization. We further determined the mobility and dimerization kinetics of TβRII on cell membrane by tracking individual receptors that were site-specifically labeled with an organic dye. In addition, after monitoring caveolae- and clathrin-mediated TβRI receptor endocytosis in living cells, we uncovered the direct fusion of clathrin- and caveolae-mediated endocytic pathways during TGF-β receptor endocytic trafficking, which could generate a new multifuntional sorting site for signal regulation.