
拉曼光谱仪
生命科学
The use of inverted microscope systems (such as the XploRA INV opens up the technique for characterisation of samples which cannot be analysed on upright microscopes. In addition to Raman analysis and 3D fluorescence FCI (Fast Confocal Imaging) standard microscope techniques can also be used on the same instrument, including epifluorescence, micro manipulators and injectors, optical tweezers, micro-fluidic cells, and specific environment enclosures.
Areas which benefit from Raman spectroscopy include:
- cell research
- disease detection
- drug design and pharmaceutical materials
- characterisation of drug-cell interactions
- microbiology and cell sorting
- cosmetics and in vivo skin analysis
- stents and implants
White light image (left), fluorescence FCI image (center) and hyperspectral fluorescence image (right) of doxorubicin in a cancer cell. The spectral image illustrates the DNA bound drug complex (DOX-DNA - red) and two cytosolic complexes of the drug (DOX – green and blue). Data courtesy of Prof. Igor Chourpa, Université de Tours, France.
Analysis results for human colonic tissue section (A) optical image of histopathologic HE stained tissue, (B) Raman mapped image illustrating intensity of the Amide I band, and (C) pseudo-colour Raman maps created by using K-means cluster analysis in which each cluster (consisting of similar spectra) is assigned to one colour, and illustrates the presence of DNA, RNA, proteins, lipids and carbohydrates. Data courtesy of Prof. Michel Manfait, Université de Reims, France.
Raman spectrum of a single bacterium, with peak labelling showing principal chemical species – (N) nucleic acids, (PA) phenylalanine, (C) carbohydrate, (P) proteins, and (L) lipids. The cluster diagram on the right hand side illustrates the capability for Raman to distinguish bacteria species – (a – red) Acinetobacter sp., (b – blue) ADP1 E. Coli DH5a, and (c – green) Pseudomonas fluorescens SBW25. Data courtesy of Dr Wei Huang, University of Sheffield, UK, and Prof. Andrew Whiteley, Centre for Ecology and Hydrology, UK.
文章
- 生命科学的光谱成像——不仅仅是一张漂亮的图片 (《光谱欧洲生命科学》增刊2005年8月- 9月)
- 生物制药成像与分析——朝着更加具体的化学构想前进 (《欧洲生物制药综述》 2005年春)
- 关于细胞成像的新视角——组合化学和空间信息 (《欧洲生物论坛》, 2005年4月)
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PPT演示
- 拉曼与荧光共焦成像表征细胞内信息
感谢法国图尔大学Igor Chourpa教授提供,展示于2010年 FACSS