生命科学

拉曼光谱具备的无损、无需标记、微米级空间分辨率等优点,使其在生命科学分析方面具有无与伦比的优越性。

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The use of inverted microscope systems (such as the XploRA INV opens up the technique for characterisation of samples which cannot be analysed on upright microscopes.  In addition to Raman analysis and 3D fluorescence FCI (Fast Confocal Imaging) standard microscope techniques can also be used on the same instrument, including epifluorescence, micro manipulators and injectors, optical tweezers, micro-fluidic cells, and specific environment enclosures.

The XploRA INV microscope for bio-spectroscopy
The XploRA INV microscope for bio-spectroscopy

Areas which benefit from Raman spectroscopy include:

  • cell research
  • disease detection
  • drug design and pharmaceutical materials
  • characterisation of drug-cell interactions
  • microbiology and cell sorting
  • cosmetics and in vivo skin analysis
  • stents and implants
  • White Light Image
  • Fluorescence FCI Image
  • Hyperspectral Fluorescence Image

White light image (left), fluorescence FCI image (center) and hyperspectral fluorescence image (right) of doxorubicin in a cancer cell.  The spectral image illustrates the DNA bound drug complex (DOX-DNA - red) and two cytosolic complexes of the drug (DOX – green and blue).  Data courtesy of Prof. Igor Chourpa, Université de Tours, France.

  • Optical image of histopathologic HE stained tissue
  • Raman mapped image illustrating intensity of the Amide I band
  • Pseudo-colour Raman maps

Analysis results for human colonic tissue section (A) optical image of histopathologic HE stained tissue, (B) Raman mapped image illustrating intensity of the Amide I band, and (C) pseudo-colour Raman maps created by using K-means cluster analysis in which each cluster (consisting of similar spectra) is assigned to one colour, and illustrates the presence of DNA, RNA, proteins, lipids and carbohydrates.  Data courtesy of Prof. Michel Manfait, Université de Reims, France.

  • Peak labelling showing principal chemical species
  • Raman spectrum of a single bacterium

Raman spectrum of a single bacterium, with peak labelling showing principal chemical species – (N) nucleic acids, (PA) phenylalanine, (C) carbohydrate, (P) proteins, and (L) lipids.  The cluster diagram on the right hand side illustrates the capability for Raman to distinguish bacteria species – (a – red) Acinetobacter sp., (b – blue) ADP1 E. Coli DH5a, and (c – green) Pseudomonas fluorescens SBW25.  Data courtesy of Dr Wei Huang, University of Sheffield, UK, and Prof. Andrew Whiteley, Centre for Ecology and Hydrology, UK.

应用报告

  • Bio01 : 人类皮肤活体拉曼测量.
  • Bio02 : 活体单淋巴细胞的表面增强拉曼光谱分析.
  • Bio03 : 单细菌细胞的拉曼分析.
  • Bio04 : 小麦核的拉曼成像.
  • Bio05 : 细胞内的表面增强拉曼光谱成像.
  • Bio06 : 高分辨表面增强拉曼光谱剖析血栓症机理.
  • Bio07 : 单细胞微生物的拉曼光谱研究
  • Bio08 : 通过监测胆固醇和游离脂肪酸的脂质沉积研究动脉粥样硬化过程

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