
Training
Fluorescence for Beginners
- Reference: FLUO1
- Dates:
- March 19-21, 2018
- May 28-30, 2018
- October 15-17, 2018
- March 19-21, 2018
- Who should attend: Beginners in Fluorescence measurements.
- Duration: 3 days (From 9 am to 5:30 pm)
- Acquire theoretical and practical knowledge on fluorescence spectrometers
- Learn how to use the software
- Acquire methodology for method development
- Learn how to set up an analytical strategy with an unknown sample
- Learn how to interpret results
- Learn how to follow the performance of the spectrometer over the time
Day 1
The Theory of the Fluorescence Principle
- Why and where fluorescence is used. Application review.
- Fluorescence Instrumentation – presentation of the different parts of the instrument and their roles.
- How to select the spectrofluorometer according to the application needs.
- Practical session – System and software presentation, Acquisition Parameters
- FluorEssence Software presentation and environment: acquisitions modules, files handling, display of data, basic functions
- Set up of acquisition parameters and single spectra measurement
Day 2
Focus on Fluorescence Lifetime
Acquisitions: Theory
- Review of the existing methods to estimate the Fluorescence lifetime
- Fluorescence lifetime instrumentation – presentation of the different parts of the instrument and their role
- How to select the spectrofluorometer according to the application needs.
- Practical session – System and software presentation, Acquisition Parameters
- DataStation Software presentation and environment: Acquisitions modules, file handling, display of data, basic functions
- Fluorescence decay analysis
Day 3
Focus on Specific Measurements
- Polarization and anisotropy: theory and applications
- Matrix scan: Excitation Emission Matrix benefits (EEM)
- Time-resolved Emission Spectra (TRES): How to estimate the emission spectra during the deexcitation process
- Practical session:
- Polarization acquisitions: parameters and spectral analysis
- How to run an anisotropy decay and what information that can be extracted
- TRES: Set up of acquisition parameters and data treatment
- Telephone: + 33 (0) 1 69 74 72 00
- Fax: + 33 (0) 1 69 09 07 21
- Email: training.hfr@horiba.com