• Reference: PSA2
  • Dates:

    • March 14, 2018
    • October 9, 2018

  • Who should attend: Users equipped with a DLS analyzer, Camsizer P4 or Camsizer XT
  • Duration: 1 day (From 9:00 am to 5:30 pm)
  • Learn how to set up good conditions to properly run a sample
  • How to interpret results in DLS

Dynamic Light Scattering Theory

  • Principle
  • Description of optical configuration & hardwar
  • Parameters necessary to set up and influence Program result
  • Cumulant method and histogram method
  • Results and interpretation (distribution base (Intensity, volume, number), description of typical statistical parameters (Zave, PI, mean size, mode, std)
  • Zeta potential principle (DLVO approach)
  • Henry equations (models available: Schmolukovski, Huckel…)
  • Molecular weight measurement (Debye plot)

Presentation of the unit with its different parts and accessories demonstrating associated applications


  • Installation
  • Software description for quick handling
  • How to create SOP and check reference materials
  • How to create a standard SOP

Method (SOP) set up on customer samples

Importance of sample preparation

Choice of Analytical Conditions and Optimisation

  • Media RI and viscosity set up
  • Detector angle selection / attenuation filters
  • Correlator set up
  • Measurement acquisition time
  • Model of computation
  • Result display configuration (Intensity base, volume base, etc.)

Results interpretation (fit optimization, and data evaluation and treatment)

Data exportation and setup and recomputation

Hands on with Common Samples such as :

  • Diluted and concentrated samples
  • Broad size distribution samples
  • Very small sample sizes (< 10nm)
  • Proteins approach
  • Emulsions
  • Powders approach

Maintenance of First Level