Fluoreszenz Spektroskopie


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  • Aqualog®, the only simultaneous absorbance and fluorescence system, measures CDOM (colored dissolved organic matter) in water 100 times faster than previous fluorescence methods...Read more
  • World's First 100MHz Picosecond Diode Source With USB Interface and Plug-and-Play Interchangeable Heads ...Read more



HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrumental response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF. DAS6, the latest version of our decay analysis software, uses algorithms based on those proven over the years by users of our industry-leading v4.2 software. These algorithms have been repackaged using ActiveX technology to produce a modular 32-bit Windows™ application similar in structure to v4.2. The Foundation license comprises the main application workspace, and allows reconvolution analysis with up to five exponentials. Additional components include anisotropy, batch analysis, lifetime distribution, micellar quenching and energy transfer. For more information about these components see below.


HORIBA Scientific DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing the opportunity for fine-tuning of parameters where necessary. The DAS6 lifetime fitting module contains reconvolution analysis with up to 5 exponentials, statistical and file manipulation utilities. Batch Mode
Batch mode analyzes datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring. This module features full reconvolution with up to five exponentials and shift iteration. Parameters can be held common between datasets. All data and results are stored in a single data file and results are automatically tabulated for use in external spreadsheet applications. Lifetime distribution
Fits a distribution of decay times and distribution plus additional exponential. Fit to exponential series
Exponential series analysis of up to 30 terms. Time-resolved Anisotropy
Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components. Each model parameter, including shift, can be fixed at a pre-determined value or optimized as part of the parameter fitting. The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse. FRET energy transfer
2-D and 3-D FRET-type energy transfer with optional additional exponential component. Yokota-Tanimoto energy transfer
Yokoto-Tanimoto energy transfer with optional additional exponential component. Micellar quenching
Micellar quenching kinetics. Global analysis
Global analysis of up to 5 exponential components and 100 decay curves.

Frequently Asked Questions For v4.2 Users

Will my existing software protection device work with v6.6?
DAS6 does not require a software protection device ("dongle"). Like most other commercial software packages, DAS6 is distributed under a single-user license agreement, and requires a unique registration key to be entered during installation. Can I still use the files created using my v4.2 software?
Yes. Although DAS6 introduces a new file structure, it also supports data files in v4.2 format. What's more, it is now much easier to import and export data in other file formats such as ASCII.

System Requirements

The following PC specifications are required to run DAS6. 1 Gb RAM, 1 Gb HD space, DVD drive, SVGA (1024x768) graphics with minimum 256 color support, mouse or other pointing device, Windows™ XP or later. Windows XP recommended.


Only HORIBA Scientific offers the following functionality in one easy to use package...

  • Proven fit algorithms with optional reconvolution of IRF (prompt)
  • Shift iteration - compensates for wavelength and quantisation shifts
  • Unique grid-search algorithm for superior chi-squared minimization -- fewer false minima than Marquardt
  • 16k maximum data range
  • "Recommend" feature automatically fills in initial estimates for fit parameters
  • Simplified file handling -- new .DAS file format stores data and analyzes in one file
  • Store up to 50 analyzes in each .DAS file
  • Open .DAS files stored anywhere on your PC simply by clicking on them
  • Intuitive user interface allows you to compare analyzes with just one mouse click
  • Interactive pan and zoom functions allow you to quickly locate distortions in your data
  • Windows™ printing support for hard copy
  • Windows™ clipboard support allows exchange of data and graphics with other applications
  • User preferences are automatically remembered
  • Modular licensing
  • Full 32-bit and 64-bit implementation (no 16-bit legacy modules) - not a DOS front end
  • Designed for Windows™ XP or later (XP/Vista/7)

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