
Particle Characterization
Top 10 Practices for DLS Sizing
- Filtering diluents so that the suspending liquid has no “extra” particles.
- Ensuring that long-range electrostatic interactions are not affecting your results.
- Ensuring that particle-particle collisions are not affecting your results.
- Covering your samples so no dust falls in from the air.
- Confirming that the autocorrelation function is smooth before examining size results.
- Confirming repeatability of Z-average size before examining size distributions.
- Ensuring your glassware is clean and dust-free.
- Ensuring that the sample has reached thermal equilibrium with the sample chamber in the instrument so that the correct viscosity is used for size calculations.
- Ensure that your sample size distribution doesn’t have a tail with particles over 10 microns in diameter.
- Filtering suspensions of very small particles (diameter < 50 nm).
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