Do I need to recover the bound molecules for MS analysis?

The coupling between SPRi and MALDI-MS (Matrix-assisted laser desorption-ionization-Time of flight=MALDI-ToF) is very simple. In fact, it is possible to perform the two analyses on the same SPRi-Slide.

Following the SPRi interaction using our systems (using MS compatible conditions, i.e. buffer, chemistry …) the SPRi-slide is removed from the SPRi system. Matrix deposition and enzymatic digestion occur on each individual spot. Then the SPRi-slide is placed on the MALDI plate holder and mass analysis occurs directly on the surface of the chip. No long procedure of elution which can cause material loss or sample contamination is necessary.

Please refer to the following articles and application notes for more information:

MS Coupling