
Training
SPRi - OpenPlex
- Reference: SPRi1
- Dates:
- February 12-14, 2018
- July 2-4, 2018
- Who should attend: OpenPlex users or people wishing to acquire an OpenPlex
- Duration: 3 days (From 9 am to 5:30 pm)
- Learn how to optimize the experimental conditions according to the interaction to be analyzed
- Learn how to make biochip spotting
- Master the use of OpenPlex
- Learn how to analyze a kinetic data
Day 1
- Introduction to kinetics of interaction: What is a «good» kinetic?
- Which biochip to choose according to the molecules to immobilize?
- How to optimize spotting (concentrations, buffers, reference, ...)?
- The important experimental parameters to be tested for SPRi (pH, stroke buffer, temperature, ...)
- Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
- Case studies
Day 2
- Choose the right surface chemistry as a function of immobilized ligands
- Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
- Master the different steps to program a spotting matrix*
- Perform spottings
Day 3
- Optimize the SPRi experimental conditions according to the interaction to be analyzed
- Master the use of OpenPlex
- Two models of interactions will be studied:
- Study of a first interaction (protein / protein)
- Analysis of the data, and description of the different analysis software.
- Study of a second interaction (DNA / DNA)
*The choice of the spotter used must be defined when registering
- Telephone: + 33 (0) 1 69 74 72 00
- Fax: + 33 (0) 1 69 09 07 21
- Email: training.hfr@horiba.com