Training

  • Reference: SPRi1
  • Dates:

    • February 12-14, 2018
    • July 2-4, 2018

  • Who should attend: OpenPlex users or people wishing to acquire an OpenPlex
  • Duration: 3 days (From 9 am to 5:30 pm)

  • Learn how to optimize the experimental conditions according to the interaction to be analyzed
  • Learn how to make biochip spotting
  • Master the use of OpenPlex
  • Learn how to analyze a kinetic data

Day 1

  • Introduction to kinetics of interaction: What is a «good» kinetic?
  • Which biochip to choose according to the molecules to immobilize?
  • How to optimize spotting (concentrations, buffers, reference, ...)?
  • The important experimental parameters to be tested for SPRi (pH, stroke buffer, temperature, ...)
  • Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
  • Case studies

Day 2

  • Choose the right surface chemistry as a function of immobilized ligands
  • Optimize experimental immobilization conditions (spotting buffer, pH, ligand concentration, duration of needle rinses / dryings, etc.)
  • Master the different steps to program a spotting matrix*
  • Perform spottings

Day 3

  • Optimize the SPRi experimental conditions according to the interaction to be analyzed
  • Master the use of OpenPlex
  • Two models of interactions will be studied:

    • Study of a first interaction (protein / protein)
    • Analysis of the data, and description of the  different analysis software.

  • Study of a second interaction (DNA / DNA)

*The choice of the spotter used must be defined when registering