ViewSizer 3000

Overview

The ViewSizer™ 3000 is a true breakthrough in Nanoparticle Tracking Analysis.

As with conventional NTA, the instrument visualises scattering from individual nanoparticles in suspension. This data is then used to determine particle movement and infer particle size using the Stokes Einstein relationship. Then, by using the known illuminated and imaged sample volume, particle number concentration is readily determined. Thus, from a single measurement, two critical pieces of information are determined: particle size distribution and particle concentration.

But the ViewSizer™ 3000 offers much more.

By incorporating three variable light sources (blue, green and red) the instrument is able to select the optimum conditions for any sample analysis, overcoming the limitations of NTA when analysing polydisperse sample and enabling a much larger range of particle sizes to be visualised.

Furthermore, by introducing the sample in easy-to-use (and clean) cuvette, the ViewSizer™ 3000 is able to repeatedly ‘analyse and stir’, giving a much more reproducible result. And because the sample is viewed in a vertical orientation, it is perfect for visualising processes such as protein aggregation or crystal dissolution.

So whether you’re using NTA for the analysis of exosomes or other microvesicles or for complex nanoparticle kinetic processes, the ViewSizer™ 3000 is a breakthrough technology, bringing better analysis to your laboratory.

Features

High resolution particle size distribution and visualisation
Wide range of particle sizes in the same sample
Multiple light sources
Perfect for monitoring nanoparticle kinetics

Manufactured by HORIBA Scientific

Specifications

Particle Size Range: 10 nm to 15 μm
Typical Sample Volume: 350 µL to 1 mL
Typical Sample Concentration: 5 x 10^6 to 2 x 10^8 particles/mL*
Sample Temperature Range (Controlled): 10 °C to 50 °C, ± 0.1 °C
Dimensions: 55 cm W x 66 cm D x 35 cm H
Mass: 27 kg
Operating Environment: 15 °C to 30 °C with < 85% RH

* Sample Dependent
Specifications are subject to change without notice.

Measurement Techniques

The system characterizes nanoparticles by analyzing their thermal motion (Brownian motion) and larger, micron-sized particles by analyzing gravitational settling. The system leverages innovative illumination and detection techniques that enable video recording of scattered light from wide-ranging sizes of individual particles simultaneously.

Particles are illuminated and the particle scattering is imaged by a microscope objective. These images are captured by video. The obtained video shows the motion of each individual nanoparticle. By taking advantage of modern video cameras and computer speed, the motion of each particle is tracked to determine the diffusion coefficient, and, from that, the size of each particle.