Measurement of Calcium in Milk and Milk Beverages

LAQUAtwin is a series of pocket ION meters. Using Ion Selective Electrode (ISE) technology, they are available for measuring Conductivity, Calcium, Nitrate, Potassium, Sodium, Salt concentration and pH measurement. Using just a tiny amount of sample, the LAQUAtwin proprietary flat sensors can quickly and accurately measure the values of the chemical parameters in the field.

Introduction

It is often necessary to determine the calcium content of milk and milk beverages. This may be done by atomic absorption spectroscopy (AA) or inductively coupled plasma atomic emission spectroscopy (ICP). Alternatively, by ionizing protein-bound calcium using an acidizing pretreatment, the LAQUAtwin Ca2+ can be used to measure the total amount of calcium easily.

The LAQUAtwin Ca2+ meter is used as check to determine the Calcium content of milk products before selling to consumers. This is an easy, quick method to check the amount of calcium in lactic products.

Method

Pretreatment procedure (for milk)

Acidify the milk with hydrochloric acid (HCl) to a pH of between 4.3 and 4.6 (which can be determined by use of the LAQUAtwin pocket pH meter). Wait for the solution to precipitate and use the liquid part of the sample for the test. A small sample of the solution is placed on the sensor of the LAQUAtwin Ca2+  and measured. To repeat sampling, wash with diluted soap water and pat dry with a paper tissue.

Pretreatment procedure (for milk beverages such as lactic drinks)

Acidify the lactic drink with hydrochloric acid (HCl) and use the LAQUAtwin pH meter to confirm it has a pH of about 2. Then, dilute this sample and add tris-hydroxy-aminomethane so that the solution has a pH value between 4.3 and 4.6. Wait for the solution to precipitate and use the liquid to sample. A small sample of the solution is placed on the sensor of the LAQUAtwin Ca2+  and measured. To repeat sampling, wash with diluted soap water and pat dry with a paper tissue.

Results and Benefits

The use of accurate Calcium ion testing in controlling the quality and calcium content of lactic products ensures that consumers are accurately able to gauge their calcium intake. This is especially beneficial for those who are lactose intolerant.

The LAQUAtwin Ca2+ pocket meter is small and compact; convenient to carry around the marketplace for easy on-site testing. Its easy-to-use interface is simple for anyone to use the LAQUATwin Ca2+ pocket meter.

1 Internal study by HORIBA labs, 2013

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