This application note shows how binding kinetics of single-domain antibodies can be studied with SPRi technology. QVQ produces single-domain antibodies from camelids (sdAbs or VHH). Like conventional Abs, sdAbs are able to bind specific epitopes with high binding affinity. The small size of sdAbs allows for enhanced tumor penetration and fast blood clearance, features that are favorable for in vivo imaging applications. Here, the binding of the anti-HER2 sdAb Q17c to recombinant HER2 protein was assessed using Surface Plasmon Resonance imaging (SPRi). An optimization of immobilization conditions was performed for Q17c and its specific antigen HER2 using a single SPRi-Biochip.
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