FRET with a HORIBA Jobin Yvon Phosphorimeter

Förster resonance energy transfer (FRET)

FRET with a HORIBA Jobin Yvon Phosphorimeter

A phosphorimeter integral to the FluoroMax®-P spectrofluorometer or as the FL-1042 accessory on the Fluorolog® system can uncover important information on various systems of chemical and biochemical
interest by revealing luminescence data normally masked by intense but rapid fluorescence.

This Technical Note describes an example of Förster resonance energy transfer (FRET) from a peptide-terbiumcomplex donor to a fluorescein acceptor, using the HORIBA Jobin Yvon phosphorimeter. The peptide in the complex absorbs light at 280 nm, and the terbium phosphoresces at 485 nm, where fluorescein dye absorbs. The fluorescence of fluorescein can be observed when the sample is excited at 280 nm, using our phosphorimeter accessory.

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