Visualizing Dental Caries Using Fluorescence Lifetime Microscopy

Teeth are naturally fluorescent and changes in their composition, caused by decay for example, affect their fluorescence behavior. The physical form and scattering nature of teeth can make simple measurements of fluorescence intensity problematic. The use of time‐resolved fluorescence microscopy provides a quick and visual means of analysis, assisted by the fact that the fluorescence lifetime is an absolute measure. Fluorescence lifetime imaging (FLIM), providing both spatial and lifetime information, is well suited to characterize tooth structure and investigate tooth decay.

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