SPRi-Continuous Flow Microspotter™ (SPRi-CFM)

SPRi CFM MM Picture Fr

Automatic printer for biomolecules


The SPRi-CFM is an automatic printer that uses flow deposition for printing biomolecules. Samples are cycled over the surface and captured from solution, leading to higher biomolecule density, better spot uniformity and improved assay sensitivity. The printhead uses a three-dimensional network of microchannels that allows for the flow of solutions over a substrate area. The printhead provides a seal to the substrate and confines the solution to the area of the individual spot, completely eliminating background signal and the possibility of cross-contamination.





Bench top printer that uses flow to immobilize biomolecules

  • Easy to use
  • Prints onto SPRi-Biochips™, SPRi-Slides™ and standard glass slides
  • Capture and deposition of molecules from complex samples
  • No cross-contamination
  • Increased spotting reproducibility and uniformity
  • Enhanced assay sensitivity


  • Print method: Flow printing
  • Number of samples: Prints 48 samples at a time in a 4x12 matrix. Up to 96 samples can be printed on a SPRi-Biochip ™ or a SPRi-Slide ™
  • Sample types: Prints from dilute samples to crude media
  • Spots size: 400 microns squares with a pitch of 875 microns
  • SPRi-Slide™ or SPRi-Biochip ™ capacity: 1 SPRi-Slide™ or 1 SPRi-Biochip™
  • Glass slide capacity: 1 standard glass slide
  • Source plate capacity: 2 standard microtiter plates (96 or 384 wells)
  • System cleaning: Automated cleaning between samples and at shutdown
Antibody-Antigen Specific interaction
Antibody-Antigen Specific interaction
HORIBA provides the pharmaceutical industry, biotech companies and academic research laboratories with high performance instrumentation based on SPRi technology (Surface Plasmon Resonance imaging). To demonstrate the power of this technology, we carried out an experiment based on an antibody-antigen interaction. Original surface chemistry combined with an electrochemical process allows the rapid coupling of biomolecules to the gold layer (polypyrrole co-polymerisation) on the top of a glass prism.
Detection of birch pollen allergen in the air
Detection of birch pollen allergen in the air
The purpose of this proof of concept is to show that Surface Plasmon Resonance imaging (SPRi) is a suitable technique to detect allergens. Compared with other techniques, such as ELISA or Luminex, which are based on colorimetric or fluorescence detection, this technology does not require the labeling of antibodies.
Real time detection of lymphocytes with SPRi
Real time detection of lymphocytes with SPRi
Validation of the activity of G-protein-coupled receptors (GPCRs) using SPRi
Validation of the activity of G-protein-coupled receptors (GPCRs) using SPRi

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