DuoScan™ Macro and Sub-Micron Raman Mapping

DuoScan Raman Accessory for Fast Imaging

Raman Accessory for fast imaging

The DuoScan™ technology enables the ultimate flexibility for Raman imaging, including easy sub-micron Raman imaging, adjustable laser spot sizes, and 100% coverage of a sample for macro-mapping.

Segment: Scientific
Division: Molecular and Microanalysis
Base product
Manufacturing Company: HORIBA France SAS


The innovative DuoScan™ Imaging system extends the imaging capabilities of HORIBA Scientific’s Raman instruments from sub-micron to macro-scale mapping. Generating confocal images becomes faster, easier and more flexible, from the deep UV to the IR.

The DuoScan™ Imaging technology available on the instruments of the LabRAM Series introduces a new imaging mode, based on a combination of scanning mirrors that scan the laser beam across a pattern chosen by the operator: a line for linear profiles, or an area for two-dimensional mapping.  It can be used to generate a rastored "macro laser spot" for large area mapping; alternatively, its 50 nm step piezo controllers are ideally suited for Raman mapping of sub-micron features.

How DuoScan works – the three modes

  1. Averaging mode

In averaging mode the laser spot is continuously scanned across a user-defined square surface

Comparison between standard point measurement and DuoScan averaging mode

Comparison between standard point measurement and DuoScanTM averaging mode with corresponding laser spot size and power densities on sample

2. Step-by-Step mode

In step-by-step mode a sample area is selected and the sample is scanned point by point across the chosen area.

Mapping without moving the sample with 50 nm step size

Mapping without moving the sample with 50 nm step size

3. Macro-mapping mode

Macro-mapping mode enables large sample surfaces to be raster-scanned to record an average spectrum across the whole surface, ideal for component distribution or to locate contaminents.

Macro-mapping with full sample coverage using DuoScan with motorized stage

Macro-mapping with full sample coverage using DuoScanTM with motorized stage

DuoScan™ Application Examples

Example 1 – Pharmaceutical Tablet

In this example a 12.5 mm by 12.5 mm pharmaceutical tablet is analysed using a DuoScan™ macro-pixel for fast large area mapping – this ensures that the entire surface area of the tablet is analysed, without ‘stepping over’ any small features.  Subsequently high resolution point-by-point mapping using the DuoScan™ piezo controlled mirrors allows a detailed image of microscopic features to be visualised.

Example 1 – Pharmaceutical Tablet

Example 2 – Carbon Nanotubes

Isolated SWCNTs aligned on a Silicon sample. The colors correspond to the integrated intensity of the Si band (red) and of the respective G bands of the CNTs (green and blue).

Example 2 – Carbon Nanotubes

DuoScan™ was used in macro-mapping mode to find the isolated CNTs. A high-definition image of the CNT was then obtained with DuoScan™ in step-by-step micro-mapping mode. The apparent width of the CNT is a convolution of the tube diameter with the laser spot size.

Sample courtesy of Dr Kalbac, Heyrovský Institute, Prague, Czech Republic

Other applications

  • Large area component mapping (contaminant screening, survey scans, etc.)

  • Chemical imaging at sub-micron scale

  • Raman analysis and imaging of photosensitive biological samples

  • Mapping on large and heavy samples that cannot be moved easily

  • Prevention of photodegradation of organic materials when working with UV excitation

  • Fast and uniform scanning over both small and large areas
  • Map area in step-by-step mode: 30 µm x 30 µm with a 50X microscope objective
  • Compatible with true confocal microscopes for high axial resolution
  • Operating spectral range from 220 nm to 1600 nm
  • Scanning step sizes down to 50nm
What is DuoScan?
Size 2.05 MB


Do you have any questions or requests? Use this form to contact our specialists.

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