Fluorescence – LED Phosphorescence Light Sources – SpectraLED

Innovative and affordable pulsed light source for phosphorescence measurements from deep UV to NIR

The SpectraLED is a novel light source designed specifically for the measurement of phosphorescence lifetimes.  These phosphorescence sources are based on LED technology and the emission wavelengths range from the deep UV to the NIR.

Segment: Scientific
Division: Molecular and Microanalysis
Base product
Manufacturing Company: HORIBA Scientific

The addition of a SpectraLED source to your HORIBA Scientific TCSPC system adds phosphorimeter capability and enables the measurement of luminescence lifetimes ranging from picoseconds to seconds in one compact system.

The SpectraLED is a modern approach to measuring longer lived luminescence decays.  Traditionally, phosphorescence lifetime measurements are excited using a xenon flashlamp.  Xenon lamps are broadband sources and offer complete wavelength coverage from the deep UV to the NIR region.  For applications where continuous wavelength tunability is not required, SpectraLEDs are a convenient alternative with the following advantages:

  • Operation at higher repetition rates not limited by capacitor charging times
  • Software control of pulse duration and repetition rate to optimally excite the sample under investigation
  • No afterglow, permitting easier interpretation of lifetimes shorter than 100us
  • Silent operation

 

SpectraLEDs can be used for measuring the long lifetimes of materials such as lanthanides, photovoltaics, minerals, security inks and singlet oxygen.

Features

  • Low cost LED-based sources for phosphorescence measurements
  • Connects directly into FluoroHub and DeltaHub for "plug-and-play" operation
  • Suitable for FluoroCube, Tempro, Fluorolog-TCSPC and FluoroMax-TCSPC systems
  • Wavelengths available from 265nm to 1275nm
  • Optical pulses from 100ns to milliseconds with sharp on-off transitions
  • Software control of the pulse rate and duration allows for optimisation of the pulse to suit time range
  • Mechanically and optically interchangeable with NanoLED and DeltaDiode sources
  • Each source contains adjustable collection optics and bayonet mounting
SOURCEPeak wavelength (nm)Typical Spectral FWHM (nm)
S-265265 +/- 10nm10
S-280280 +/- 10nm10
S-290290 +/- 10nm10
S-295295 +/- 10nm10
S-340340 +/- 10nm10
S-350350 +/- 10nm14
S-370370 +/- 10nm15
S-390390 +/- 10nm20
S-415415 +/- 10nm20
S-460460 +/- 10nm30
S-495495 +/- 10nm30
S-525525 +/- 20 mn35
S-590590 +/- 10nm15
S-605605 +/- 15nm20
S-625625 +/- 10nm25
S-740740 +/- 10nm25
S-830830 +/- 10nm50
S-970970 +/- 20nm50
S-12001200 +/- 20nm90
S-12751275 +/- 20nm90

Notes:

(1) Specifications and appearance are subject to change without notice.

(2) Please contact us if you require a wavelength not appearing on the list above.

Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
Measuring PL Upconversion Spectra and Lifetimes of Lanthanide-Doped Nanoparticles
Upconverting lanthanide-based nanomaterials exhibit a unique fluorescence anti-Stokes shift, which enables them to convert NIR wavelength excitation into visible shorter wavelength emissions (NIR to UV-Vis).
Characterizing Lanthanides in Glasses for Optical Applications
Characterizing Lanthanides in Glasses for Optical Applications
Glasses are essential materials with a multitude of uses and many forms. In the area of optoelectronics there is an interest to modify the glass composition to favor the incorporation of lanthanide elements.
Upconversion of Lanthanide-containing glasses using DD‐980L excitation
Upconversion of Lanthanide-containing glasses using DD‐980L excitation
The phenomenon of upconversion is an optical process that takes in lower energy (longer wavelength) photons and emits higher energy (shorter wavelength) photons.
Measurement of carrier lifetime in perovskite for solar cell applications
Measurement of carrier lifetime in perovskite for solar cell applications
Hybrid perovskite photovoltaics (PV) show promise because of their good efficiencies, which can be around 20%. Along with their PV characteristics, perovskite materials exhibit a high degree of radiative recombination.
Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
Monitoring Whole Leaf Fluorescence Using Time‐resolved Techniques
Light incident on a leaf can be absorbed by chlorophyll to commence the photosynthetic cycle. Excess energy can be liberated as heat or by emission of fluorescence and this can be used to assess the efficiency of the photosynthetic process. Because of the Kautsky effect, the amount of fluorescence emanating from photosystem II (PSII) is not constant. There is an initial rise, upon illumination followed by a decrease caused by PSII closing down, as it is unable to transfer electrons. There can also be a contribution from PSI to the overall fluorescence. Here we employ different time‐resolved fluorescence techniques to demonstrate the types of measurement that can be performed on a whole leaf.
Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
Stopped flow time‐resolved fluorescence study of serum albumin – curcuminoid binding
Rapid mixing accessories to perform stopped flow measurements have found application in characterizing interactions and reactions occurring in solution. Reactants are expelled from syringes, mixed and injected into a flowcell.
Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
Dye‐protein binding monitored in a microliter volume using timeresolved fluorescence
The potential health benefits stemming from the antioxidant activity of curcumin, commonly found in turmeric (Curcuma longa L), has attracted the interest of several research groups.
Visualizing dental caries using fluorescence lifetime microscopy
Visualizing dental caries using fluorescence lifetime microscopy
Teeth are naturally fluorescent and changes in their composition, caused by decay for example, affect their fluorescence behavior.
The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
The Measurement of Singlet Oxygen Lifetime Sensitized using Rose Bengal
The study of singlet oxygen (1O2) is of interest, principally, as it is a highly reactive species. It can be produced by photosensitisation, usually of a molecule such as a dye or porphyrin. Thus, by the appropriate selection of sensitiser, the presence of oxygen and light, 1O2 can be selectively generated. From a biological aspect it has the ability to damage and destroy cells, which has lead to interest in its use as an anticancer agent in photodynamic therapy (PDT).
Visualizing local viscosity using fluorescence lifetime microscopy
Visualizing local viscosity using fluorescence lifetime microscopy
Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence
Effect of temperature on HSA structure inferred using timeresolved room-temperature phosphorescence
To access intrinsic amino acids, such as tryptophan, as probes, the UV excitation wavelengths for pulsed phosphorescence measurements have long been the preserve of low-repetition-rate gas-filled lamps or larger laser systems. Recent developments have enabled the use of interchangeable semiconductor diodes, with their inherent ease of use. SpectraLEDs provide spectral coverage from the UV to the near-IR.
Plasmon enhancement of protein fluorescence by silver nanostructures
Plasmon enhancement of protein fluorescence by silver nanostructures
The use of metal surfaces in conjunction with fluorescence molecules employing a plasmon effect, sometimes referred to as metal enhanced fluorescence, can be advantageous because of the possible enhancement of photophysical properties. For example, the emission intensity of the fluorophore, can be improved.
Investigating photocleavage using time‐resolved emission spectra
Investigating photocleavage using time‐resolved emission spectra
The choice of protecting group is of crucial importance in the success of many steps in organic synthesis and the manipulation of polyfunctional molecules, since they can prevent the formation of undesired side products and reactions.
Time‐resolved luminescence of security inks from the UV to NIR
Time‐resolved luminescence of security inks from the UV to NIR
The use of security features, such as luminescent inks, has increased significantly in an attempt to prevent fraud and counterfeiting of materials and goods.
Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
Elucidating Local Viscosity Using Fluorescence Lifetime Measurements
Certain fluorescent molecules, known as molecular rotors, can be employed to estimate the local (nanoscale) viscosity in microheterogeneous systems by measurement of their fluorescence lifetime. This can be advantageous over the usual fluorescence anisotropy method, as the measurement is simpler and faster to perform. This is demonstrated using the HORIBA Scientific TemPro fluorescence lifetime system to monitor the gelation of silica produced using the sol‐gel technique.

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