What is Fluorescence? The term fluorescence refers to one type of luminescence. Luminescence, broadly defined, is light emission from a molecule. There are several types of luminescence.
The Fluorolog®-3 is a unique, modular system which allows the researcher to interchange a wide range of accessories to correspond precisely with the characteristics of a given sample. From analysis of steady-state or molecular dynamics to IR probes, the Fluorolog®-3 comes equipped with a wide range and limitless configuration of accessories to enhance the accuracy and speed of it’s performance. The Fluorolog®-3 series enables you to customize your spectrofluorometer’s performance to meet your research needs.
It can also perform (TCSPC) Time Correlated Single Photon Counting lifetime measurements like the FluoroMax-4 and QM800.
Fully modular and customizable, the Fluorolog®-3 can stand alone as the centerpiece of your laboratory or interface with other instruments to multiply the capabilities of your lab.
* Finding it difficult to compare sensitivity specifications? We explain how to test your spectrofluorometer.
FluorEssence™ for Windows® sets a new standard for fluorescence software. FluorEssence™ merges spectrofluorometer data-acquisition directly to the famous processing power of Origin® software.
Simple clicks let you select a scan type, accessory or bring back a complete experimental set-up.
Revolutionize the Way You Operate Your Spectrofluorometer:
FluorEssence™ also includes the powerful processing and data-management tools of Origin® to streamline your laboratory’s operation.
Data-viewing, plotting, processing and management are all available, including extensive import and export facilities, view manipulation and creation, arithmetic applications, peak-picking and integration, data-management and much more!
HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrument response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF.
DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing for fine-tuning of parameters where necessary. Analysis modules include reconvolution analysis of multi-exponential models plus analysis of more specialised fluorescence decay processes as detailed below.
Analysis for up to 5 exponentials.
Batch mode supports datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring. It features full reconvolution with up to five exponentials and shift iteration.
Perform global analyses of up to 5 exponential components and 100 decay curves.
Two methods are available for performing lifetime distribution analysis. The first method employs a “Top Hat” distribution function which allows a single lifetime distribution to be fitted in conjunction with an additional discrete lifetime. The second method is an optional module which will perform a Non-Extensive Decay distribution which allows up to five lifetime distributions to be modelled.
Exponential series analysis of up to 30 terms. This allows parameterisation of otherwise difficult data.
2D and 3D Foerster type energy transfer with an optional exponential component.
Yokoto-Tanimoto energy transfer taking into account diffusion with an optional exponential component.
Micellar quenching kinetics can help determine the surfactant aggregation number.
Exciplex analysis follows formation of an excited state species.
Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components. Each model parameter, including shift, can be fixed at a pre-determined value or optimised as part of the parameter fitting. The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.
DataStation is the latest measurement environment for time-domain fluorescence and is supplied with all HORIBA Scientific lifetime systems.
Lifetime systems are made up of various modules and components, including pulsed light sources, PMTs, monochromators, motorized polarizers, focusing optics, MCAs etc. DataStation integrates the control of all these components into one intuitive, mouse-driven Windows environment.
Lifetime systems can perform a variety of measurement functions, such as fluorescence decay measurement, phosphorescence decay measurement, time-resolved anisotropy measurements, steady-state spectra, time-resolved emission scan (TRES), temperature scans and more. These measurements are now routine using DataStation. Measurement templates are provided to quickly set up the instrument for the required measurement, and automated data acquisition is performed using computer controlled components.
Acquired data can be saved in a variety of formats including the .das format, HORIBA Jobin Yvon IBH v4.2, and ASCII, printed to your Windows printer, exported or copied to the clipboard for manipulation using a third-party application.
DataStation can be purchased separately for use on non-HORIBA Scientific systems, and we are happy to add custom functionality and interfacing (e.g. to additional monochromator types, motorized polarizer holders etc).
The DataStation software comes into its own when combined with a FluoroHub.
DataStation evolves continually as new functionality is added in response to customer suggestion. The following is a partial list:
Temperature control support
The following PC specification are required to run DataStation. Measurement functionality depends on spectrometer hardware attached. Basic operation requires a supported MCA card or FluoroHub. Contact HORIBA Scientific for more information.
1 Gb RAM, 1 Gb HD space, DVD drive, 1024x768 graphics with minimum 256 color support, mouse or other pointing device. Windows™ XP or later (XP/Vista/7). Windows™ XP recommended.
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