XelPleX

XelPlex

Fully automated label-free molecular interaction analysis machine One System for Molecule Screening & Crude Sample Analysis

 

XelPleX is a high-performance and fully automated instrument for the analysis of label-free biomolecular interactions in a multiplex format.

The array-based format of the sensor chips allows you to monitor up to several hundreds interactions simultaneously and to accelerate your research.

The optimized fluidic system is designed to give you full kinetic profiles within minutes and help you make the right decisions quickly and with confidence.

XelPleX is powered with EzSuite, our new software suite for simple use and advanced data analysis.

Segment: Scientific
Division: Molecular and Microanalysis
Manufacturing Company: HORIBA France SAS

Automated SPR imaging system for label-free assays:

  • Multiplex Detection of several hundred interactions
  • Label-Free protein, peptide, DNA samples
  • Real-Time monitoring of kinetic curves
  • Difference Image Display giving you a direct view of reactions as the experiment unfolds
  • Fast Total Assay Time - typical assays in less than 10 minutes
  • Temperature control
  • Sample recycling for maximum binding
  • Sample recovery function
  • Direct subtraction of the negative control

 

 

 

  • Sample volume: typically 200 μL
  • Sample concentration: 150 ng/mL (100-1000 kDa) to 5 μg/mL (4-20 kDa)
  • Sample molecular weight: ≈150 Da
  • Limit of detection: 5 pg/mm2
Production of a single domain antibody Q17c directed against recombinant HER2 protein and its binding study by Surface Plasmon Resonance imaging technology
Production of a single domain antibody Q17c directed against recombinant HER2 protein and its binding study by Surface Plasmon Resonance imaging technology
This application note shows how binding kinetics of single-domain antibodies can be studied with SPRi technology. QVQ produces single-domain antibodies from camelids (sdAbs or VHH). Like conventional Abs, sdAbs are able to bind specific epitopes with high binding affinity. The small size of sdAbs allows for enhanced tumor penetration and fast blood clearance, features that are favorable for in vivo imaging applications. Here, the binding of the anti-HER2 sdAb Q17c to recombinant HER2 protein was assessed using Surface Plasmon Resonance imaging (SPRi). An optimization of immobilization conditions was performed for Q17c and its specific antigen HER2 using a single SPRi-Biochip.
Production of a rescued recombinant monoclonal antibody directed against a steroid hormone and its binding study thanks to Surface Plasmon Resonance imaging technology
Production of a rescued recombinant monoclonal antibody directed against a steroid hormone and its binding study thanks to Surface Plasmon Resonance imaging technology
A monoclonal antibody (mAb) highly specific to a steroid hormone (undisclosed) of 290 Daltons was analyzed thanks to SPRi technology. This application note highlights the sensitivity of the XelPleXTM system for the detection of small molecules.
Streamlined SPRi-MS coupling to detect and identify a kinase in Cell lysate using the DARPins as binders
Streamlined SPRi-MS coupling to detect and identify a kinase in Cell lysate using the DARPins as binders
DARPins (Designed Ankyrin Repeat Proteins) are a class of non-immunoglobulin binders. Thanks to their specificity and robustness, they allow a multitude of novel, so far unfeasible applications. Surface Plasmon Resonance imaging (SPRi) is a powerful label-free technique that enables real-time target detection. Combining SPRi to massspectrometry (MS) allows biomolecules identification using their unique peptide mass fingerprint. In the past this combination was cumbersome and time-consuming. This application note shows how a protein kinase (RPS6KA2), a potential drug target is detected and identified using a hyphenated On-Chip SPR-MS coupling protocol, leading to saving time and money.
Validation of the activity of G-protein-coupled receptors (GPCRs) using SPRi
Validation of the activity of G-protein-coupled receptors (GPCRs) using SPRi
The Detection of an Ultralow Molecular Weight Enzyme Inhibitor using the XelPleX system
The Detection of an Ultralow Molecular Weight Enzyme Inhibitor using the XelPleX system
Rapid couplig of SPRi and proteinchip
Rapid couplig of SPRi and proteinchip
Real time detection of lymphocytes with SPRi
Real time detection of lymphocytes with SPRi
SPRi Imaging of oligosaccharides proteins interactions
SPRi Imaging of oligosaccharides proteins interactions
Protein-Peptide interactions studies with SPRi
Protein-Peptide interactions studies with SPRi
Description of the advantages of combining the use of peptide chips with direct label-free detection as achieved by SPR imaging (SPRi).
Monitoring of interactions between aptamers and human IgE by SPRi
Monitoring of interactions between aptamers and human IgE by SPRi
Label-free Ligand Fishing in Human Plasma
Label-free Ligand Fishing in Human Plasma
Interaction between immobilized peptides and protein from sera on Cystamine / Glutaraldehyde functionalized biochip
Interaction between immobilized peptides and protein from sera on Cystamine / Glutaraldehyde functionalized biochip
How to quantify a protein in different crude samples in one run, using the XelPleX
How to quantify a protein in different crude samples in one run, using the XelPleX
Detection of birch pollen allergen in the air
Detection of birch pollen allergen in the air
Antibody-Antigen Specific interaction
Antibody-Antigen Specific interaction

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