Training course

Training

Fluorescence for Beginners

Overview

  • Reference: FLUO1
  • Dates:
    • March 18-20, 2019
    • May 20-22, 2019
    • October 14-16, 2019
  • Who should attend: Beginners in Fluorescence measurements.
  • Duration: 3 days (From 9 am to 5:30 pm)

Objectives

  • Acquire theoretical and practical knowledge on fluorescence spectrometers
  • Learn how to use the software
  • Acquire methodology for method development
  • Learn how to set up an analytical strategy with an unknown sample
  • Learn how to interpret results
  • Learn how to follow the performance of the spectrometer over the time

Program

Day 1

The Theory of the Fluorescence Principle

  • Why and where fluorescence is used. Application review.
  • Fluorescence Instrumentation – presentation of the different parts of the instrument and their roles.
  • How to select the spectrofluorometer according to the application needs.
  • Practical session – System and software presentation, Acquisition Parameters
    • FluorEssence Software presentation and environment: acquisitions modules, files handling, display of data, basic functions
    •  Set up of acquisition parameters and single spectra measurement

 

Day 2

Focus on Fluorescence Lifetime
Acquisitions: Theory

  • Review of the existing methods to estimate the Fluorescence lifetime
  • Fluorescence lifetime instrumentation – presentation of the different parts of the instrument and their role
  • How to select the spectrofluorometer according to the application needs.
  •  Practical session – System and software presentation, Acquisition Parameters
    •   DataStation Software presentation and environment: Acquisitions modules, file handling, display of data, basic functions
    •  Fluorescence decay analysis

Day 3

Focus on Specific Measurements

  • Polarization and anisotropy: theory and applications
  • Matrix scan: Excitation Emission Matrix benefits (EEM)
  • Time-resolved Emission Spectra (TRES): How to estimate the emission spectra during the deexcitation process
  • Practical session:
    • Polarization acquisitions: parameters and spectral analysis
    • How to run an anisotropy decay and what  information that can be extracted
    • TRES: Set up of acquisition parameters and data treatment

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