Training course

Training

Fluorescence for Beginners

Overview

  • Reference: FL1
  • Dates:
    • March 16-17, 2020
    • June 8-9, 2020
    • October 12-13, 2020
  • Who should attend: Beginners in Fluorescence measurements.
  • Duration: 3 days (From 9 am to 5:30 pm)

Objectives

  • Acquire theoretical and practical knowledge on fluorescence spectrometers
  • Learn how to use the software
  • Acquire methodology for method development
  • Learn how to set up an analytical strategy with an unknown sample
  • Learn how to interpret results
  • Learn how to follow the performance of the spectrometer over the time

Program

Day 1

The Theory of the Fluorescence Principle

  • Why and where fluorescence is used. Application review.
  • Fluorescence Instrumentation – presentation of the different parts of the instrument and their roles.
  • How to select the spectrofluorometer according to the application needs.
  • Practical session – System and software presentation, Acquisition Parameters
    • FluorEssence Software presentation and environment: acquisitions modules, files handling, display of data, basic functions
    •  Set up of acquisition parameters and single spectra measurement

 

Day 2

Focus on Fluorescence Lifetime
Acquisitions: Theory

  • Review of the existing methods to estimate the Fluorescence lifetime
  • Fluorescence lifetime instrumentation – presentation of the different parts of the instrument and their role
  • How to select the spectrofluorometer according to the application needs.
  •  Practical session – System and software presentation, Acquisition Parameters
    •   DataStation Software presentation and environment: Acquisitions modules, file handling, display of data, basic functions
    •  Fluorescence decay analysis

Day 3

Focus on Specific Measurements

  • Polarization and anisotropy: theory and applications
  • Matrix scan: Excitation Emission Matrix benefits (EEM)
  • Time-resolved Emission Spectra (TRES): How to estimate the emission spectra during the deexcitation process
  • Practical session:
    • Polarization acquisitions: parameters and spectral analysis
    • How to run an anisotropy decay and what  information that can be extracted
    • TRES: Set up of acquisition parameters and data treatment

Our trainers are experts in each technique. They will provide trainings advice and guidance to make the most of your HORIBA Scientific instrument. You will gain confidence and experience in the analysis of your samples.

Certificates are given to every attendee for every course.

All trainings are held in France or at your location and are conducted in  French or English.

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