Training course


Particle Size Analyzer: Light Scattering Technique

These trainings are held in France or at your location and are conducted in French or English.


  • Reference: PSA2
  • Dates:
    • March 11, 2020
  • Who should attend: Users equipped with a DLS analyzer, Camsizer P4 or Camsizer XT
  • Duration: 1 day (From 9:00 am to 5:30 pm)


  • Learn how to set up good conditions to properly run a sample
  • How to interpret results in DLS


Dynamic Light Scattering Theory

  • Principle
  • Description of optical configuration & hardwar
  • Parameters necessary to set up and influence Program result
  • Cumulant method and histogram method
  • Results and interpretation (distribution base (Intensity, volume, number), description of typical statistical parameters (Zave, PI, mean size, mode, std)
  • Zeta potential principle (DLVO approach)
  • Henry equations (models available: Schmolukovski, Huckel…)
  • Molecular weight measurement (Debye plot)

Presentation of the unit with its different parts and accessories demonstrating associated applications


  • Installation
  • Software description for quick handling
  • How to create SOP and check reference materials
  • How to create a standard SOP

Method (SOP) set up on customer samples

Importance of sample preparation

Choice of Analytical Conditions and Optimisation

  • Media RI and viscosity set up
  • Detector angle selection / attenuation filters
  • Correlator set up
  • Measurement acquisition time
  • Model of computation
  • Result display configuration (Intensity base, volume base, etc.)

Results interpretation (fit optimization, and data evaluation and treatment)

Data exportation and setup and recomputation

Hands on with Common Samples such as :

  • Diluted and concentrated samples
  • Broad size distribution samples
  • Very small sample sizes (< 10nm)
  • Proteins approach
  • Emulsions
  • Powders approach

Maintenance of First Level

Our trainers are experts in each technique. They will provide trainings advice and guidance to make the most of your HORIBA Scientific instrument. You will gain confidence and experience in the analysis of your samples.

Certificates are given to every attendee for every course.


Do you have any questions or requests? Use this form to contact our specialists.