Training course


Raman Microscopy for Beginners


  • Dates:
    • February 11-13, 2019
    • May 13-15, 2019
    • June 24-26, 2019
    • October 7-9, 2019
    • November 18-20, 2019
  • Who should attend:Users of HORIBA Scientific Raman spectrometers
  • Duration:  3 days (From 9 am to 5 pm)


  • Acquire theoretical and practical knowledge on
    Raman spectrometers
  • Learn how to use the software
  • Learn methodology for method development and
    major analytical parameters
  • How to set up an analytical strategy with an
    unknown sample
  • How to interpret results
  • Learn how to follow the performances of the
    Raman spectrometer over the time.


Day 1

  • The theory of the Raman principle
  • Raman Instrumentation
  • Practical session – System and softwarepresentation, Acquisition Parameters:

             - LabSpec 6 presentation and environment: user accounts, file handling, display of data, basic functions
             - Set up of acquisition parameters and single spectra measurement
             - Templates & Reports

Day 2

  • Analysis of Raman spectra
  • Practical session: Raman spectrum measurement and Database Search

              - Optimization of the parameters: how to chose the laser, the grating, the confocal hole, the laser power
              - How to use the polarization options
              - Library Search using KnowItAll software
              - How to create databases

           Raman imaging

  • How to make a Raman image (1D, 2D and 3D)
  • Data evaluation: cursors, CLS fitting, peakfitting
  • Image rendering, 3D datasets
  • Fast mapping using SWIFT XS

Day 3

Data processing

  •  Processing on single spectra and datasets
  •  Baseline correction
  •  Smoothing
  •  Normalization
  •  Spectra subtraction, averaging
  •  Data reduction
  •  Methods
  •  Practical exercises


Customer samples: Bring your own samples!


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