Training course


Raman Microscopy for Beginners


  • Reference: RAM1
  • Dates:
    • February 10-12, 2020
    • May 11-13, 2020
    • September 21-23, 2020
    • November 16-18, 2020
  • Who should attend: Users of HORIBA Scientific Raman spectrometers
  • Duration:  3 days (From 9 am to 5:30 pm)


  • Acquire theoretical and practical knowledge on
    Raman spectrometers
  • Learn how to use the software
  • Learn methodology for method development and
    major analytical parameters
  • How to set up an analytical strategy with an
    unknown sample
  • How to interpret results
  • Learn how to follow the performances of the
    Raman spectrometer over the time.


Day 1

  • The theory of the Raman principle
  • Raman Instrumentation
  • Practical session – System and software presentation, Acquisition Parameters:
    • LabSpec 6 presentation and environment: user accounts, file handling, display of data, basic functions
    • Set up of acquisition parameters and single spectra measurement
    • Templates & Reports

Day 2

  • Analysis of Raman spectra
  • Practical session: Raman spectrum measurement and Database Search
    • Optimization of the parameters: how to chose the laser, the grating, the confocal hole, the laser power
    • How to use the polarization options
    • Library Search using KnowItAll software
    • How to create databases
  • Raman imaging
    • How to make a Raman image (1D, 2D and 3D)
    • Data evaluation: cursors, CLS fitting, peakfitting
    • Image rendering, 3D datasets
    • Fast mapping using SWIFT XS

Day 3

  • Data processing
    • Processing on single spectra and datasets
    • Baseline correction
    • Smoothing
    • Normalization
    • Spectra subtraction, averaging
    • Data reduction
    • Methods
    • Practical exercises
  • Customer samples: Bring your own samples!


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