Training course

Training

SPRi - Customer Training on their Applications

Overview

  • Reference: SPRi3
  • Dates:
    • March 11-14, 2019
    • September 10-13, 2019
  • Who should attend:XelPleX or OpenPlex users People wishing to acquire an XelPleX or an OpenPlex.
    For this training the attendees should be from the same team 
  • Duration: 3 days (From 9 am to 5 pm)

Objectives

  • Learn how to optimize experimental conditions in function of interactions to analyze
  • Learn how to spot the biochip with the spotter defined by the user
  • Master the use of the SPRi system, defined beforehand by the user
  • Learn how to analyze data

Program

 

Day 1

  • Which biochip to choose according to the molecules to immobilize?
  • How to optimize spotting (concentrations, buffers, reference, ...)?
  • The important experimental parameters to be tested in SPRi (pH, running buffer, temperature,...)
  • Advice to be applied during the realization of the experiment (duration of kinetics, flow rate, regeneration, ...)
  • Establishment of an experimental protocol

 

Day 2

  • Choose suitable surface chemistry for immobilized ligands
  • Perform spotting based on the previously defined protocol
  • Advice about the maintenance of the instrument

 

Day 3

  • Carry out the SPRi experiment as described in the protocol
  • Master the use of the chosen SPRi device (XelPleX or OpenPleX)

 

Day 4

  • Reminder on interaction kinetics
  • Software use
  • Data analysis

                                                                          

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