The LMNE detection principle is based on the Double Hydrodynamic Sequential System "DHSS" flowcytometry.
Two characteristics for each cell passing through this system are determined: volume and
absorbency (HORIBA Medical patent).
1. The cells go through the aperture one by one (1)
to be counted and measured by electrical current
(impedance changes). This is the resistive
2. Then each one crosses the light beam that
arrives at a 0° angle (2) determining the cells
A part of the light is absorbed by the cell
another part is deviated by the cell structure
and the rest passes through or around the
It is the light passing through the cells that is
measured (optical transmission). The absorbency
is determined from this optical transmission.